Page 44 - ITPS-6-2

INNOSC Theranostics

and Pharmacological Sciences

ORIGINAL RESEARCH ARTICLE
Evaluation of genotoxicity of (4-fluorophenyl)

thiazolidin-4-one in CHO-K1 cells

1
1†
Jhansi Mamilla , Kalpana Javvaji 1,3† , Kavya Lekha Sunkara ,
2
2,3
Umesh B. Kosurkar , Ravindra M. Kumbhare * and Sunil Misra *
1,3
1 Genetic Toxicology Lab, Applied Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka,
Hyderabad, India
2 Department of Fluoro-Agrochemicals, CSIR-Indian Institute of Chemical Technology, Tarnaka,
Hyderabad, India
3 Academy of Scientific and Innovative Research, Ghaziabad, India

Abstract
4-thiazolidinones are five-membered heterocyclic ring compounds with diverse
pharmacological impacts. In a previous study, we reported a series of newly
synthesized derivatives of 4-thiazolidinones with different functional groups,
which exhibited anticancer activity against ovarian (SKOV3) and cervical (HeLa)
cancer cell lines. Among these derivatives, (4-fluorophenyl) thiazolidin-4-one (4-TH)
demonstrated potent cytotoxic activity against SKOV3, with an IC value of 12.3 µM.
50
However, it was also found to be extremely toxic to normal cells (CHO-K1) with an
† These authors contributed equally
to this work IC of 7.5 µM. Before considering its use in cancer research, it is crucial to gain a
50
*Corresponding authors: comprehensive understanding of its potential genotoxic effects on normal cells. In
Sunil Misra this study, we aimed to assess the in vitro cytogenetic toxicity of 4-TH using normal
(smisra@iict.res.in) Chinese hamster ovary cells (CHO-K1). Referring to the IC of 4-TH, we selected
Ravindra M. Kumbhare 50
(kumbhare@iict.res.in) three sub-lethal concentrations (2, 5, and 7.5 µM) and treated CHO-K1 cells for 24 h
(one cell cycle duration) to estimate its dose-dependent induction of chromosome
Citation: Mamilla J, Javvaji K, aberrations, and examine the effect of 4-TH on cell division, micronucleus induction
Sunkara KL., et al., 2023,
Evaluation of genotoxicity of potential and cell cycle arrest properties following standard protocols. The results
(4-fluorophenyl) thiazolidin-4- showed that 4-TH was highly toxic to normal cells, as all three sublethal concentrations
one in CHO-K1 cells. INNOSC caused a statistically significant increase in the number of chromosomal aberrations
Theranostics and Pharmacological
Sciences, 6(2):0618. (P < 0.001), formation of micronuclei (P < 0.01), and changes in the rate of cell division
https://doi.org/10.36922/itps.0618 (mitotic index) (P < 0.05) compared to control. In addition, there was a significant
Received: April 18, 2023 increase in the number of cells in the G1 phase, indicating that all concentrations
of 4-TH tested induced apoptosis. The evaluation of the cytotoxic, clastogenic, and
Accepted: July 18, 2023
aneugenic properties of 4-TH, a potent cytotoxic agent, will undoubtedly provide
Published Online: August 7, 2023 critical information for determining its safety and potential as an anticancer drug.
Copyright: © 2023 Author(s).
This is an Open Access article
distributed under the terms of the Keywords: Cytotoxicity; Chromosome aberration; Mitotic index; Micronucleus; Cell cycle
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium,
provided the original work is
properly cited. 1. Introduction
[1,2]
Publisher’s Note: AccScience Heterocyclic compounds hold significant pharmacological importance . Recently, there
Publishing remains neutral with has been increasing industrial and biological interest in the chemistry of 4-thiazolidinones
regard to jurisdictional claims in [3]
published maps and institutional due to their diverse bioactive properties . Thiazolidinones are five-membered heterocyclic
affiliations. compounds with a sulfur atom at position 1, a nitrogen atom at position 3, and a carbonyl group

Volume 6 Issue 2 (2023) 1 https://doi.org/10.36922/itps.0618