Page 40 - ITPS-6-2

INNOSC Theranostics and
Pharmacological Sciences Residual versus curative antimalarial tests

Table 12. In vivo repository antimalarial screening of the aqueous and organic extracts of Chromolaena odorata in Plasmodium
berghei‑infected mice
Plant Extract Dose D Parasite D Chemoprophylaxis D Parasite D Chemoprophylaxis Mean survival
6
8
8
6
part (mg/kg) density (%) (%) density (%) (%) time (%)
Leaves H O 100 2.78±2.67 15.41 36.52±2.76 23.61 11.75±0.96
2
250 5.47±0.98 13.57 40.06±2.40 16.19 10.50±0.58
400 4.13±1.27* 34.78 49.40±7.64 −3.34 10.00±0.82
Positive Chloroquine 5 4.40±1.05* 30.52 42.77±4.56 10.53 10.75±0.96
control
Negative Distilled H O 0.2 mL 6.33±1.30 0.00 47.80±2.42 0.00 10.50±1.00
2
control
Leaves Organic 100 7.23±1.26 14.91 29.59±4.72 15.68 12.60±2.19
250 4.21±2.12* 50.47 28.31±3.86* 40.57 12.20±1.64
400 3.72±2.03* 56.21 36.17±1.40* 34.01 12.20±1.64
Positive Chloroquine 5 5.87±1.59* 30.92 38.53±5.11 15.15 12.20±1.64
control
Negative Tween 80 0.2 mL 8.50±1.00 0.00 45.41±3.07 0.00 11.60±0.55
control
Notes: *Indicates significant difference from control, P≤0.05. Chemosuppression: ≥60%, high activity; 30 – 60%, moderate activity; <30%, weak
[13]
activity; 0%, inactivity .

14 45
Aqueous
Organic
12 40 Curative
Repository
35
10 30
Number of extracts 8 6 Number of extracts 25

4 20
15
2
10

0
High Moderate Weak No activity 5
Figure 3. In vivo antiplasmodial activities of the selected medicinal plant
extracts in repository and curative tests. 0
High (60%) Moderate Weak (‹ 30%) No activity
saponins, flavonoids, tannins, and terpenes [39-41] . These (30-60%) (0%)
phytochemical compounds increase the level of oxidative Figure 4. In vivo antiplasmodial activities of the selected medicinal plant
molecules in the cells, thereby inhibiting protein synthesis in extracts in repository and curative tests.
the parasites . This mechanism could nullify the oxidative
[42]
[43]
damage induced by the malarial parasite. It also suggests antiplasmodial effects of these phytochemicals . These
that the antiplasmodial activity of the ethanolic leaf extract findings corroborate the reported antiplasmodial activity
of N. latifolia may be attributed to the antioxidant and of the plant and support further evidence for its traditional

Volume 6 Issue 2 (2023) 13 https://doi.org/10.36922/itps.0300