Page 55 - ITPS-6-2

INNOSC Theranostics and
Pharmacological Sciences Liquid herbal preparations

of the 10 dilution was spread-plated on the well-dried Table 1. Information of selected samples
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surface of Sabouraud dextrose agar and incubated at 25°C
Expiry date
Manufacturing
(room temperature) for 4 days to allow for possible growth Code date (month/year) (month/year) Batch number
of fungi [2,15,18,19] . Each sample was inoculated in triplicates.
HM1 06/19 06/21 LVCM 000290 LHC
2.2.4.3. Test for E. coli HM2 05/19 05/22 HL-0015V
One milliliter of each sample was pipetted and subjected HM3 06/18 06/21 001
to a 10-fold serial dilution to 10 . An inoculum of 0.1 mL HM4 06/19 06/22 00G05
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of the 10 dilution was spread-plated on the well-dried HM5 03/19 03/22 0319
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surface of MacConkey agar. The Petri dishes containing HM6 08/18 08/22 FPVL0018
the spread-plated MacConkey agar were inverted and HM7 11/19 11/21 TX97
incubated at 37°C for 24 h to allow for possible growth of
E. coli [2,15,18,19] . Each sample was inoculated in triplicates. HM8 10/19 10/21 00342
HM9 02/19 02/21 MAMI 01/2019
2.2.4.4. Test for S. typhi HM10 10/19 10/21 0052
One milliliter of each sample was pipetted and subjected HM11 01/19 01/24 004
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to a 10-fold serial dilution to 10 . An inoculum of 0.1 mL HM12 11/17 11/22 004
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of the 10 dilution was spread-plated on the well-dried HM13 08/19 7/21 MC/08/19
surface of bismuth sulfite agar in a petri dish. The petri HM14 03/19 03/21 ADT/1116
dishes containing the spread-plated bismuth sulfite agar
were inverted and incubated at 37°C for 24 h to allow HM15 10/19 10/21 589
for possible growth of S. typhi [2,15,18,19] . Each sample was
inoculated in triplicates. Table 2. Seal integrity test results for sampled herbal
preparations
2.2.4.5. Test for Pseudomonas aeruginosa
One milliliter of each sample was pipetted and subjected to Sample code Observation
a 10-fold serial dilution to 10 . An inoculum of 0.1 mL of HM1 No leak observed
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the 10 dilution was spread-plated on the well-dried surface HM2 No leak observed
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of cetrimide agar. The petri dishes containing the spread- HM3 No leak observed
plated cetrimide agar were inverted and incubated at 37°C HM4 No leak observed
for 24 h to allow for possible growth of P. aeruginosa [2,15,18,19] . HM5 No leak observed
Each sample was inoculated in triplicates.
HM6 No leak observed
2.2.4.6. Test for S. aureus HM7 No leak observed
One milliliter of each sample was pipetted and subjected HM8 No leak observed
to a 10-fold serial dilution to 10 . An inoculum of 0.1 mL HM9 No leak observed
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of the 10 dilution was spread-plated on the well-dried HM10 No leak observed
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surface of mannitol salt agar. The petri dishes containing HM11 No leak observed
the spread-plated mannitol salt agar were inverted and HM12 No leak observed
incubated at 37°C for 24 h to allow for possible growth of
S. aureus [2,15,18,19] . Each sample was inoculated in triplicates. HM13 No leak observed
HM14 No leak observed
3. Result HM15 No leak observed
Manufacturing dates, expiry dates, and batch numbers
obtained from the sampled liquid herbal products together The results of the presence or otherwise of specific
with their designated codes are summarized in Table 1. The microorganisms in the sampled liquid herbal preparations
shelf life of all sampled products was longer than 1 year are shown in Table 3. HM1, HM2, HM3, HM8, and HM10
from the date of purchase. were not contaminated with microorganisms based on

Results of the seal integrity test on the primary package the nutrient agar results, and these results were confirmed
of the sampled herbal liquid preparations are summarized by the absence of specific microorganisms in the various
in Table 2. None of the sampled herbal liquid preparations selective media used. Samples HM4, HM5, and HM9 were
failed the sealed integrity test. contaminated with both fungi and E. coli, while samples

Volume 6 Issue 2 (2023) 3 https://doi.org/10.36922/itps.0425