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258 Yoksa, et al. | Journal of Clinical and Translational Research 2024; 10(4): 256-262
Table 1. Effects of burn wound treatment on red blood cell parameters in albino rats treated with 1% silver sulphadiazine (SSD) or 5% resveratrol
(RSV) topical ointments or no treatment (NT)
Parameter Groups (n=5) Day
5 8 21
PCV (%) Control 46.40±4.39 a 44.60±3.21 a 45.60±3.97 a
1% SSD 46.80±2.39 a 46.20±4.15 a 47.60±4.15 a
5% RSV 45.40±3.78 a 43.40±3.97 a 45.40±3.36 a
NT 44.60±3.50 a 43.40±3.97 a 45.20±3.03 a
Hb (ɡ/dL) Control 17.06±2.72 a 16.40±1.39 a 16.30±0.84 a
1% SSD 15.10±1.19 a 16.00±1.66 a 16.20±1.68 a
5% RSV 16.58±1.52 a 15.90±1.24 a 16.00±1.00 a
NT 16.42±0.89 a 14.00±2.72 a 16.12±0.38 a
RBC (× 10 µL) Control 7.53±0.85 a 7.30±0.31 a 7.37±0.51 a
6
1% SSD 7.93±0.86 a 7.62±0.44 a 7.85±0.59 a
5% RSV 7.26±0.54 ab 7.10±0.82 a 7.33±0.59 a
NT 6.80±1.25 b 5.48±1.42 b 7.15±0.61 a
MCV (fL) Control 61.76±1.41 a 61.01±2.28 a 61.84±1.99 a
1% SSD 59.32±3.58 a 60.56±2.80 a 60.59±2.31 a
5% RSV 62.55±2.52 ab 61.39±4.10 a 62.01±4.15 a
NT 65.64±1.25 b 84.39±27.23 b 63.37±4.16 a
MCH (pɡ) Control 22.86±5.15 a 22.49±2.29 a 22.16±1.12 a
1% SSD 19.18±2.20 a 20.96±1.09 a 20.61±0.72 a
5% RSV 22.99±3.13 ab 22.74±4.06 a 21.97±2.50 a
NT 24.21±1.05 b 25.96±3.43 a 22.65±1.77 a
MCHC (ɡ/dL) Control 36.94±6.08 a 37.00±5.20 a 35.91±3.00 a
1% SSD 32.29±5.52 a 34.63±1.53 b 34.02±1.40 a
5% RSV 36.81±5.18 a 36.94±5.07 a 35.41±3.66 a
NT 36.89±1.60 a 32.76±8.09 a 35.77±2.06 a
Note: Superscripts ( and ) define the significance of differences in the mean (for each parameter) between groups (row) for each day (column). The same superscript applied down a
a
b
column indicates non-significant differences (P>0.05), while a different superscript in the same column indicates a significant difference (P<0.05). For instance, on day 8 (RBC), the means
for the control, 1% SSD, and 5% RSV are not significantly different from each other (denoted by ), but they are significantly different from the mean of the NT group (denoted by ).
a
b
Abbreviations: PCV: Packed cell volume; Hb: Hemoglobin; RBC: Red blood cell; MCV: Mean corpuscular volume; MCH: Mean corpuscular hemoglobin; MCHC: Mean corpuscular
hemoglobin concentration.
mean packed cell volume and hemoglobin concentration across significant decrease compared to the 1% SSD group (P > 0.05).
all the groups are not significantly different (P > 0.05) at 5, 8, and The mean absolute neutrophil count in the 5% RSV group
21 days PT (DPT). The mean total red blood cell count (tRBC) decreased significantly compared to the control, 1% SSD, and
is significantly lower in the 5% RSV group compared to the NT group at 5, 8, and 21 DPT, suggesting neutropenia induced
control and 1% SSD group at 5 DPT (P < 0.05), but significant by 5% RSV treatment. There was slight monocytopenia in rats
differences were not observed at 8 and 21 DPT (P > 0.05). The treated with 5% RSV compared to the 1% SSD group at 8 DPT
mean value of MCV in the 5% RSV group was significantly (P < 0.05). At 5 and 21 DPT, no statistical differences were
lower (P < 0.05) compared to the no treatment (NT) group recorded in all groups. No significant differences were observed
at 5 and 8 DPT, but no differences were observed at 21 DPT in mean absolute lymphocyte count at 5, 8, and 21 DPT between
(P > 0.05). The mean MCH was significantly different in 5% groups (P > 0.05).
RSV-treated and NT rats at 5 and 8 DPT (P < 0.05), but no
significance was observed at 21 DPT. Similarly, no significant 3.3. Effects of burn wound treatment on the activities of serum
difference was observed for mean MCHC in all groups at 5, 8, ALT, AST, and ALP
and 21 DPT (P > 0.05). The effects of BW treatment on liver function are summarized
3.2. Effects of burn wound treatment on white blood cell in Table 3. Elevated serum ALT level was observed in the 5%
parameters RSV group at 5 DPT (P < 0.05), suggesting that 5% RSV did
not prevent hepatocellular damage at the early stages of BW. As
The effect of RSV topical ointment on leukocyte parameters the wound healed, a significant decrease in mean serum ALT
is summarized in Table 2 as the mean ± standard deviation. The activity was observed in the 5% RSV group from 5–21 DPT
mean tWBC count is not significantly different at 5 and 8 DPT (P < 0.05). Serum AST activity was elevated in the 1% SSD,
(P > 0.05). At 21 DPT, the 5% RSV group displayed a non- 5% RSV, and NT groups compared to the control group at 5 and

DOI: https://doi.org/10.36922/jctr.24.00019