Page 15 - JCTR-10-6

Journal of Clinical and Translational Research 2024; 10(6): 325-333

Journal of Clinical and Translational Research

Journal homepage: http://www.jctres.com/en/home

ORIGINAL ARTICLE

Establishment and characterization of patient-derived high-grade glioma

cell lines, and validation of their tumorigenicity in murine

xenograft model

Natália Barreto , Valquiria Aparecida Matheus , Ingrid Mayara Cavalcante Trevisan , Thaís Tuasca Jareño ,
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Giovanna Marques Antunes , Julio Cesar de Moraes , Liana Verinaud *, Jean Gonçalves de Oliveira ,
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José Carlos Esteves Veiga , João Luiz Vitorino-Araujo , Catarina Rapôso *
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1 Faculty of Pharmaceutic Sciences, University of Campinas, Campinas, São Paulo, Brazil, Division of Neurosurgery, Department of Surgery,
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Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil, Imunocel Laboratory, Campinas, São Paulo, Brazil, Department of
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Structural and Functional Biology, Biology Institute, University of Campinas, Campinas, São Paulo, Brazil
ARTICLE INFO ABSTRACT
Article history: Background: Diffuse high-grade gliomas (HGGs) include the most aggressive types of brain
Received: June 14, 2024 tumors. Despite current treatment options, which include a combination of surgery, radiotherapy,
Accepted: October 8, 2024 and chemotherapy, the prognosis remains catastrophic. Patients diagnosed with glioblastoma (GB),
Published Online: December 5, 2024 the most aggressive HGG, have a median survival of <2 years. Cancer cell lines represent an
essential tool in cancer research. Once established, these cells can be used to investigate tumor
Keywords: biology and conduct drug screening trials, contributing to the development of new therapeutic
High-grade gliomas options for patients with glioma.
Cancer cell lines Aim: The aim of the study was to establish and characterize three new HGG cell lines obtained
Human primary cell line from different patients and validate their tumorigenicity in a murine xenograft model.
Glioblastoma Methods: The three tissue samples were immunohistochemically and molecularly classified as
Tumorigenicity astrocytoma IDH-mutant, Grade 3 (C03); GB IDH-wildtype, grade 4 (N07); and astrocytoma IDH-
mutant, Grade 3 (L09). These were cultured until the tenth passage for culture establishment. Cell
*Corresponding authors: morphology was accessed by light microscopy and phalloidin labeling. To characterize the cell
Catarina Rapôso lines, GFAP labeling was performed. Xenograft murine models were used to investigate whether
Faculty of Pharmaceutic Sciences, University the cell lines retained their tumor-forming ability. Cells from murine tumors were recultured, and
of Campinas, Campinas, São Paulo, Brazil. morphological evaluation was performed by histopathological analysis.
Email: raposo@unicamp.br Results: The three HGG lines were successfully established, and GFAP positivity confirmed
Liana Verinaud their astrocytic origin. Morphologically, the cells presented a fusiform or polygonal shape, with
Department of Structural and Functional accelerated growth throughout the passages. All three cell lines developed tumors after induction
Biology, Biology Institute, UNICAMP, of the xenograft model, and the subculture of these tumors revealed a morphology similar to that
Campinas, São Paulo, Brazil. of the three cell lines before implantation. Histopathological analysis of the xenograft tumors
Email: verinaud@unicamp.br
confirmed the disordered tissue formation commonly found in diffuse gliomas.
© 2024 Author(s). This is an Open-Access Conclusion: The successful establishment of these cell lines and the creation of a biobank will
article distributed under the terms of the facilitate studies in drug development and glioma tumorigenesis.
Creative Commons Attribution-Noncommercial Relevance for Patients: The established cell lines will be utilized in assays to analyze glioma
License, permitting all non-commercial use, tumorigenesis and in screening for novel drug candidates, contributing to the development of new
distribution, and reproduction in any medium, treatments for these patients.
provided the original work is properly cited.

1. Introduction
Diffuse high-grade gliomas (HGGs) are a heterogeneous group of tumors that originate
from glial or glial precursor cells and are among the most aggressive and prevalent

DOI: http://doi.org/10.36922/jctr.24.00028