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Microbes & Immunity Anti-mouse CXCR5 monoclonal antibody
A
B
Figure 3. Flow cytometry analysis of mCXCR5-expressing LN229 cells using Cx Mab-3 and L138D7. LN229/mCXCR5 (A) and LN229 (B) cells were
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treated with 0.01 – 10 µg/mL of Cx Mab-3 (black line), L138D7 (black line), or control blocking buffer (filled gray). Then, cells were treated with anti-rat
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IgG conjugated with Alexa Fluor 488. Fluorescence data were collected using the SA3800 Cell Analyzer.
Abbreviation: IgG: Immunoglobulin G.
not others. We confirmed the expression of each receptor and investigated its application in flow cytometry to detect
(Figure 6B). mCXCR5 (Figures 2-5). We confirmed the specificity
of Cx Mab-3 among eighteen CC, CXC, CX3C, and XC
4. Discussion chemokine receptors (Figure 6). Therefore, Cx Mab-3 can
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In this study, we developed a novel anti-mCXCR5 mAb recognize mCXCR5-expressing cells with high specificity
(clone Cx Mab-3) using N-terminal peptide immunization in in vivo experiments. We assessed the reactivity of
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Volume 2 Issue 1 (2025) 106 doi: 10.36922/mi.5664
