Page 116 - MI-2-1

Microbes & Immunity Anti-mouse CXCR5 monoclonal antibody

A

B

Figure 6. Flow cytometry analysis of Cx Mab-3 in CC, CXC, CX3C, and XC chemokine receptor-expressed CHO-K1 cells. (A) The eighteen CC, CXC,
5
CX3C, and XC chemokine receptor-expressed CHO-K1 cells were treated with 1 µg/mL of Cx Mab-3 (red line) or control blocking buffer (black line),
5
followed by the treatment with anti-rat IgG conjugated with Alexa Fluor 488. (B) The cells were treated with 1 µg/mL of corresponding mAbs, including
anti-CXCR1 mAb (clone 1122A), anti-CXCR2 mAb (clone SA045E1), anti-CXCR3 mAb (clone CXCR3-173), anti-CXCR4 mAb (clone L276F12), anti-
CXCR5 mAb (clone L138D7), anti-CXCR6 mAb (clone SA051D1), anti-CCR1 mAb (clone S15040E), anti-CCR2 mAb (clone EPR20844), anti-CCR3
mAb (clone J073E5), anti-CCR5 mAb (clone C Mab-2 ), anti-CCR7 mAb (clone 4B12), anti-CCR8 mAb (clone C Mab-2 ), anti-CCR9 mAb (clone
50
51
5
8
CW-1.2), anti-XCR1 mAb (clone ZET), anti-CX3CR1 (clone SA011F11) mAb, or anti-PA tag mAb (clone NZ-1) (blue line). Then, cells were treated with
corresponding secondary antibody conjugated with Alexa Fluor 488. Fluorescence data were collected using the SA3800 Cell Analyzer.
Abbreviation: IgG: Immunoglobulin G.
Volume 2 Issue 1 (2025) 108 doi: 10.36922/mi.5664