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Microbes & Immunity Brachyspira pilosicoli novel outer membrane proteins

Table 3. (Continued)
Locus identifier Protein names a Total variations Variations present on the predicted
extracellular loop region
BP951000_RS03405 Hypothetical protein 24 variations: M1, R2, L3, K4, F5, F6, None
F7, L8, I9, F10, L11, F12, L13, S14, L15,
S16, L17, Y18, T19, Q20, D21, N22, E23,
and A24
BP951000_RS00185 b Hypothetical protein 58 variations Not determined c
BP951000_RS10320 Hypothetical protein 25 variations: L18, D48, E55, F251, G285, None
E400, Y401, G402, I403, F404, T405,
K406, Q407, L408, A409, I410, S411,
F412, I413, P414, I415, N416, I417, R418,
and F419
BP951000_RS05445 DUF3575 domain-containing Six variations: K2, I7, A79, N87, H89, N87 and H89
protein and K158
BP951000_RS08300 Tia invasion determinant Three variations: L143, N156, and S200 None
BP951000_RS05490 b Tia invasion determinant 61 variations Not determined c
BP951000_RS07500 b Hypothetical protein 183 variations Not determined c
BP951000_RS01590 Hypothetical protein Three variations: V205, I215, and V221 None
BP951000_RS08295 Tia invasion determinant Six variations: N34, I49, V123, S141, N34
I144, and V167
BP951000_RS08975 TonB-dependent receptor Two variations: D32 and T371 D32
domain-containing protein
BP951000_RS06930 b Serpentine receptor domain- 135 variations Not determined c
containing protein
BP951000_RS03290 Serpentine receptor domain- None None
containing protein
BP951000_RS00765 b Serpentine receptor domain- 70 variations Not determined c
containing protein
BP951000_RS01280 Serpentine receptor domain- Five variations: V32, A83, V124, E210, E210
containing protein and T237
BP951000_RS10445 Serpentine receptor domain- G228 None
containing protein
BP951000_RS00365 Serpentine receptor domain- 11 variations: V72, Q77, I84, D156, D159, D156, D159, and A200
containing protein V168, N177, A200, T216, I222, and Y226
BP951000_RS04620 Serpentine receptor domain- Four variations: K2, E95, A140, and V194 None
containing protein
BP951000_RS04220 b Serpentine receptor domain- 218 variations Not determined c
containing protein
Notes: Protein names follow annotations in the National Center for Biotechnology Information and UniProt databases, retrieved using protein accession
a
numbers (accessed on March 28, 2024). Sequence comparison of these proteins across nine strains of Brachyspira pilosicoli revealed variations at more
b
than 40 positions. Therefore, they have been listed in Table S5. Given that sequence variations were present at more than 40 positions, we have not
c
determined whether these variations are present on the transmembrane region or loop region of the predicted proteins.
Abbreviations: DUF: Domain of unknown function; OMBB: Outer membrane β-barrel; OMP: Outer membrane protein; Trep: transcriptional regulating
protein.
switching or antigenic variation by expressing alternative Brachyspira pilosicoli VspE, BP951000_RS10215,
protein phenotypes. They may function as mediators contains a secretory signal peptide. The structural model
62
for bacterial attachment to host cells. Vsp-like proteins generated by AlphaFold 3 revealed a β-barrel architecture
64
have been identified in B. hyodysenteriae, B. pilosicoli, and consisting of 16 β-strands, with the ninth and 10 strands
th
Mycoplasma; 29,62,65 however, homologs in other bacterial longer than the others, giving an elliptical shape to the
genera remain undiscovered, highlighting their unique extracellular surface of the barrel (Figure 2B). BP951000_
role in these pathogens. RS10215 exhibited the best structural alignment with the

Volume 2 Issue 4 (2025) 89 doi: 10.36922/MI025230050