Page 54 - OR-1-3

doi: 10.1016/j.isci.2020.101432 doi: 10.1038/s41467-024-53073-z
190. Grist SM, Nasseri SS, Poon T, Roskelley C, Cheung KC. 201. Nwokoye PN, Abilez OJ. Bioengineering methods for
On-chip clearing of arrays of 3-D cell cultures and micro- vascularizing organoids. Cell Rep Methods. 2024;4(6):100779.
tissues. Biomicrofluidics. 2016;10(4):044107.
doi: 10.1016/j.crmeth.2024.100779
doi: 10.1063/1.4959031
202. Simoes-Abade MBC, Patterer M, Nicaise AM, Pluchino S.
191. Costantini I, Cicchi R, Silvestri L, Vanzi F, Pavone FS. In-vivo Brain organoid methodologies to explore mechanisms of
and ex-vivo optical clearing methods for biological tissues: disease in progressive multiple sclerosis. Front Cell Neurosci.
Review. Biomed Opt Express. 2019;10(10):5251-5267. 2024;18:1488691.
doi: 10.1364/BOE.10.005251 doi: 10.3389/fncel.2024.1488691
192. Koh I, Hagiwara M. Gradient to sectioning CUBE workflow 203. Mrza MA, He J, Wang Y. Integration of iPSC-derived
for the generation and imaging of organoids with localized microglia into brain organoids for neurological research. Int
differentiation. Commun Biol. 2023;6(1):299. J Mol Sci. 2024;25(6):3148.
doi: 10.1038/s42003-023-04694-5 doi: 10.3390/ijms25063148

193. Yang B, Treweek JB, Kulkarni RP, et al. Single-cell 204. Hyun I, Scharf-Deering JC, Lunshof JE. Ethical issues related
phenotyping within transparent intact tissue through to brain organoid research. Brain Res. 2020;1732:146653.
whole-body clearing. Cell. 2014;158(4):945-958. doi: 10.1016/j.brainres.2020.146653
doi: 10.1016/j.cell.2014.07.017 205. Lavazza A, Reichlin M. Human brain organoids: Why
194. Frolikova M, Blazikova M, Capek M, et al. Innovative sample there can be moral concerns if they grow up in the lab
preparation using alcohol dehydration and high refractive and are transplanted or destroyed. Camb Q Healthc Ethics.
index medium enables acquisition of two-channel super- 2023;32(4):1-15.
resolution 3D STED image of an entire oocyte. J Microsc. doi: 10.1017/S096318012300021X
2025;297(2):165-178.
206. Mollaki V. Ethical challenges in organoid use. BioTech
doi: 10.1111/jmi.13363 (Basel). 2021;10(3):12.
195. Renier N, Adams EL, Kirst C, et al. Mapping of brain doi: 10.3390/biotech10030012
activity by automated volume analysis of immediate early
genes. Cell. 2016;165(7):1789-1802. 207. de Jongh D, Massey EK, VANGUARD Consortium,
Bunnik EM. Organoids: A systematic review of ethical
doi: 10.1016/j.cell.2016.05.007 issues. Stem Cell Res Ther. 2022;13(1):337.
196. Pavlova IP, Shipley SC, Lanio M, Hen R, Denny CA. doi: 10.1186/s13287-022-02950-9
Optimization of immunolabeling and clearing techniques
for indelibly labeled memory traces. Hippocampus. 208. Jun Y, Lee J, Choi S, et al. In vivo-mimicking microfluidic
2018;28(7):523-535. perfusion culture of pancreatic islet spheroids. Sci Adv.
2019;5(11):eaax4520.
doi: 10.1002/hipo.22951
doi: 10.1126/sciadv.aax4520
197. Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-
Lavigne M. iDISCO: A simple, rapid method to 209. Ye S, Marsee A, van Tienderen GS, et al. Accelerated
immunolabel large tissue samples for volume imaging. Cell. production of human epithelial organoids in a miniaturized
2014;159(4):896-910. spinning bioreactor. Cell Rep Methods. 2024;4(11):100903.
doi: 10.1016/j.cell.2014.10.010 doi: 10.1016/j.crmeth.2024.100903
198. Erturk A, Becker K, Jahrling N, et al. Three-dimensional 210. Licata JP, Schwab KH, Har-El YE, Gerstenhaber JA,
imaging of solvent-cleared organs using 3DISCO. Nat Lelkes PI. Bioreactor technologies for enhanced organoid
Protoc. 2012;7(11):1983-1995. culture. Int J Mol Sci. 2023;24(14):11427.
doi: 10.3390/ijms241411427
doi: 10.1038/nprot.2012.119
211. Kim W, Gwon Y, Park S, Kim H, Kim J. Therapeutic strategies
199. Cauzzo S, Bruno E, Boulet D, et al. A modular framework
for multi-scale tissue imaging and neuronal segmentation. of three-dimensional stem cell spheroids and organoids for
Nat Commun. 2024;15(1):4102. tissue repair and regeneration. Bioact Mater. 2023;19:50-74.
doi: 10.1038/s41467-024-48146-y doi: 10.1016/j.bioactmat.2022.03.039
200. Kim D, Lim H, Youn J, Park TE, Kim DS. Scalable 212. Misra S, Moro CF, Del Chiaro M, et al. Ex vivo organotypic
production of uniform and mature organoids in a 3D culture system of precision-cut slices of human pancreatic
geometrically-engineered permeable membrane. Nat ductal adenocarcinoma. Sci Rep. 2019;9(1):2133.
Commun. 2024;15(1):9420. doi: 10.1038/s41598-019-38603-w

Volume 1 Issue 3 (2025) 28 doi: 10.36922/OR025100010