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Tumor Discovery

ORIGINAL RESEARCH ARTICLE
Investigating long noncoding RNA HA117 and

its possible regulatory network in osteosarcoma

Hongxia Chen 1† , Zhiyong Cheng 2† , Wei Wang 3 , Zhenhua Zhuang 4 ,
Xiaoping Huang 3 , and Ning Wang *
3
1 Department of Hematology, Chong Qing University Three Gorges Hospital, Chongqing, China
2 Orthopedic Trauma Unit, Chong Qing University Three Gorges Hospital, Chongqing, China
3 Department of Oncology, Chong Qing University Three Gorges Hospital, Chongqing, China
4 Chengdu Life Baseline Technology CO., LTD, Chengdu, Sichuan, China

Abstract

Osteosarcoma, a primary malignant bone tumor, is the third most common cancer in
children and adolescents under 20 years old. In recent decades, many osteosarcoma-
related molecular targets, including long noncoding RNA (lncRNA), have been
discovered or confirmed. This study aims to elucidate the gene expression and
possible regulatory mechanisms of lncRNA HA117 in osteosarcoma. To achieve this,
we downloaded 51 whole-transcriptome osteosarcoma sequencing samples from the
NCBI Sequence Read Archive database and performed bioinformatics analysis. Gene
† These authors contributed equally expression analysis of HA117 revealed no significant difference between the tumor
to this work.
and adjacent tissues. However, HA117 exhibited significant down-regulation in both
*Corresponding author: fresh and formalin‐fixed paraffin-embedded (FFPE) samples after chemotherapy. By
Ning Wang
(sxyykywsb@cqu.edu.cn) combining two target gene prediction methods, we identified 11 and 83 target genes
for HA117 in fresh and FFPE samples, respectively. Functional analysis indicated that
Citation: Chen H, Cheng Z, these target genes are mainly located in the cytoplasm and nucleus, with most related
Wang W, Zhuang Z, Huang
X, Wang N. Investigating long to protein binding. In addition, Reactome analysis demonstrated that these target
noncoding RNA HA117 and its genes participate in the regulation of multiple metabolic pathways, predominantly
possible regulatory network in in cellular responses to stress. Our findings suggest that chemotherapy may regulate
osteosarcoma. Tumor Discov.
2024;3(3):3670. downstream target genes by altering the expression of HA117, thereby inducing
doi: 10.36922/td.3670 cellular stress response. Overall, our results indicate that HA117 may not function as
Received: May 15, 2024 an oncogene but could serve as a therapeutic target in osteosarcoma.
Accepted: July 3, 2024
Keywords: LncRNA; Osteosarcoma; Differentially expressed genes; Gene Ontology
Published Online: August 7, 2024
analysis; Reactome analysis; Target gene
Copyright: © 2024 Author(s).
This is an Open-Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution, 1. Introduction
and reproduction in any medium,
provided the original work is Osteosarcoma, a primary malignant bone tumor, is particularly prevalent among
properly cited. children and adolescents under 20 years old, ranking as the third most common cancer
1-3
Publisher’s Note: AccScience among this demographic. This malignant tumor primarily affects the long bones but
Publishing remains neutral with can also affect other bones in the body. Osteosarcoma, classified as a primary bone
4
regard to jurisdictional claims in
published maps and institutional tumor, has a potential for recurrence and metastasis, possibly due to a subset of cells
affiliations. with stem cell-like characteristics maintaining the tumor’s regenerative ability. Although

Volume 3 Issue 3 (2024) 1 doi: 10.36922/td.3670