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Tumor Discovery LncRNA HA117 in osteosarcoma regulation

states, suggesting that HA117 may serve as a potential Writing – original draft: Xiaoping Huang, Hongxia Chen
therapeutic target for osteosarcoma research. This finding Writing – review & editing: Ning Wang
is consistent with previous studies in leukemia. HA117
can affect the expression of a large number of downstream Ethics approval and consent to participate
target genes through trans-regulation, involving functions Not applicable.
such as metabolic immunity and cellular stress stimulation.
Meanwhile, due to the lack of miRNA data, it is currently Consent for publication
unclear which miRNAs can adsorb HA117 and form a Not applicable.
sponge effect. Therefore, additional experiments are needed
to confirm the relationships among HA117, miRNA, and Availability of data
mRNA.
Raw data can be obtained from the NCBI SRA database
5. Conclusion under the accession number PRJNA389279.

By re-analyzing 36 fresh samples and 15 FFPE samples References
containing the lncRNA HA117 in osteosarcoma, we found
no difference in HA117 expression between the tumor and 1. Bernardini G, Laschi M, Geminiani M, Santucci A.
Proteomics of osteosarcoma. Expert Rev Proteomics.
adjacent tissues. However, HA117 exhibited significant 2014;11(3):331-343.
down-regulation before and after chemotherapy. We then
combined two methods to predict 11 and 83 target genes of doi: 10.1586/14789450.2014.900445
HA117 in fresh and FFPE samples, respectively. GO analysis 2. Chen R, Huang LH, Gao YY, Yang JZ, Wang Y. Identification
showed that these target genes are mainly located in the of differentially expressed genes in MG63 osteosarcoma
cytoplasm and nucleus, and are related to protein binding. In cells with drug-resistance by microarray analysis. Mol Med
addition, 17 genes were found to be related to extracellular Rep. 2019;19(3):1571-1580.
exosomes. Reactome analysis revealed that these genes doi: 10.3892/mmr.2018.9774
were significantly enriched in four pathways: Interferon- 3. Karadurmus N, Sahin U, Bahadir Basgoz B, Demirer T. Is
alpha/beta signaling, cellular responses to stress, cellular there a role of high dose chemotherapy and autologous stem
responses to stimuli, and cellular responses to activation. cell transplantation in the treatment of Ewing’s sarcoma and
Subsequently, survival analysis on 11 key target genes of osteosarcomas? J Buon. 2018;23(5):1235-1241.
HA117 indicated that low expression BCL7A, NISCH,
and PGD was beneficial for the survival of osteosarcoma 4. Moore DD, Luu HH. Osteosarcoma. Cancer Treat Res.
2014;162:65-92.
patients. These results suggest that HA117 may play a role in
the response to chemotherapy and is a potential therapeutic doi: 10.1007/978-3-319-07323-1_4
target in osteosarcoma. However, these transcriptome data 5. Tamari K, Hayashi K, Ishii H, et al. Identification of
are limited, and further research is needed to elucidate the chemoradiation-resistant osteosarcoma stem cells using
regulatory mechanisms between HA117 and target genes. an imaging system for proteasome activity. Int J Oncol.
2014;45(6):2349-2354.
Acknowledgments doi: 10.3892/ijo.2014.2671
None. 6. Gorlick R. Current concepts on the molecular biology of
osteosarcoma. Cancer Treat Res. 2009;152:467-478.
Funding
doi: 10.1007/978-1-4419-0284-9_27
This research was funded by the Natural Science
Foundation of Chongqing, Project number cstc2020jcyj- 7. Gorlick R, Khanna C. Osteosarcoma. J Bone Miner Res.
2010;25(4):683-691.
msxmX0966.
doi: 10.1002/jbmr.77
Conflict of interest 8. Zhou G, Shi X, Zhang J, Wu S, Zhao J. MicroRNAs
The authors declare no competing interests. in osteosarcoma: From biological players to clinical
contributors, a review. J Int Med Res. 2013;41(1):1-12.
Author contributions doi: 10.1177/0300060513475959
Conceptualization: Hongxia Chen, Ning Wang 9. Du X, Yang J, Yang D, Tian W, Zhu Z. The genetic basis for
Investigation: Zhiyong Cheng inactivation of Wnt pathway in human osteosarcoma. BMC
Methodology: Wei Wang, Zhenhua Zhuang Cancer. 2014;14:450.

Volume 3 Issue 3 (2024) 11 doi: 10.36922/td.3670