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Advanced Neurology Long-term in vivo MRI tracking of SPIO-labeled NSCs

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Figure 3. Tracing of transplanted NSCs labeled with SPIO in tMCAO mice. (A) Representative images of MRI T2-star coronal plane showing the migration
and distribution of transplanted NSCs. MRI was taken at 7, 14, 21, and 28 days after tMCAO surgery. n = 3 in each group. (B) Representative images of
MRI T2-star horizontal plane showing the migration and distribution of transplanted NSCs. MRI was taken at 7, 14, 21, and 28 d after tMCAO surgery.
n = 3 in each group. (C) Representative images of PB staining showing the distribution of iron deposits in the brain at 28 days after tMCAO surgery. Insets,
individual images from PB staining. Red arrows point to SPIO signals. Scale bar = 100 μm.
MRI: Magnetic resonance imaging, NSCs: Neural stem cells, SPIO: Superparamagnetic iron oxide, tMCAO: transient middle cerebral artery occlusion.

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Figure 4. Tracing transplanted NSCs labeled with SPIO in tMCAO mice. (A) Verification of NSCs labeling rate of synthetic rhodamine B (Rhd B)-SPIOs
in vitro. Scale bar = 50 μm. (B) Immunofluorescence evaluation of peri-infarct cortex implanted with SPIO-labeled NSCs. DAPI (blue), nucleus; GFP
(green), NSCs; SPIO-Rhd-B (red), which were coated with dextran and visualized with a rhodamine-conjugated dextran. Scale bar = 100 μm.
DAPI: 4’,6-diamidino-2-phenylindole, GFP: Green fluorescent protein, NSCs: Neural stem cells, PB: Prussian blue, Rhd B: Rhodamine B,
SPIO: Superparamagnetic iron oxide, tMCAO: transient middle cerebral artery occlusion.

Volume 1 Issue 3 (2022) 7 https://doi.org/10.36922/an.v1i3.278