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Advanced Neurology Long-term in vivo MRI tracking of SPIO-labeled NSCs

this study, we first identified the appropriate concentration Methodology: Ying Bai, Zhongqiu Zhou, Fengchao Zang,
of SPIO nanoparticles. The results showed that the amount and Guangtian Wang
of iron increased with the increase of SPIO concentration, Supervision: Ling Shen, Yuan Zhang, and Shuo Leng
demonstrating that NSCs internalized SPIO nanoparti- Writing – original draft: Ying Bai and Guangtian Wang
cles in a dose-dependent manner; thus, we chose an SPIO Writing – review & editing: Guangtian Wang and Bing Han
nanoparticle concentration of 200 μg/mL. This SPIO con-
centration had no significant effect on the proliferation and Ethics approval and consent to participate
differentiation of NSCs, providing high biological security All animal experiments were performed in accordance with
to track NSCs in the tMCAO mice model. Developing the standard guidelines for the care and use of laboratory
long-term tracking is a major problem in stem cell therapy. animals and approved by the Institutional Animal Care
A marker should first effectively bind to the stem cell and and Use Committee of the Medical School of Southeast
the signal should not disappear within a short time [33,34] . In University.
this study, SPIO nanoparticles were used to label NSCs and
were tracked by MRI at different time points, demonstrat- Consent for publication
ing that transplanted SPIO-labeled NSCs can migrate to Not applicable.
infarcted areas. Taken together, the current work based on
SPIO-based MRI imaging enables the visualization of the Availability of data
distribution, migration, and survival of transplanted NSCs
in tMCAO mice, thereby facilitating a good understanding Data can be obtained from the corresponding author
of the role of NSCs in ischemic stroke. following request.

5. Conclusion References
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in which better magnetic properties were observed 2. Herpich F, Rincon F, 2020, Management of acute ischemic
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transplanted NSCs labeled with SPIO can migrate from the
injection site to the infarcted area in vivo, thus providing 3. Tao T, Liu M, Chen M, et al., 2020, Natural medicine
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therapy toward clinical application. 4. Wang F, Tang H, Zhu J, et al., 2018, Transplanting
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Acknowledgments Cell Transplant, 27(12): 1825–1834.
None. https://doi.org/10.1177/0963689718795424

Funding 5. Jiang XC, Xiang JJ, Wu HH, et al., 2019, Neural stem
cells transfected with reactive oxygen species-responsive
This work was supported by grants from the National polyplexes for effective treatment of ischemic stroke. Adv
Natural Science Foundation of China (82104147). Mater, 31(10): e1807591.
Conflict of interest https://doi.org/10.1002/adma.201807591
6. Wechsler LR, Bates D, Stroemer P, et al., 2018, Cell therapy
The authors declare that the research was conducted in the for chronic stroke. Stroke, 49(5): 1066–1074.
absence of any commercial or financial relationships that
could be construed as potential conflicts of interest. https://doi.org/10.1161/STROKEAHA.117.018290
7. Bai Y, Ren H, Bian L, et al., 2022, Regulation of glial function
Author contributions by noncoding RNA in central nervous system disease.

Conceptualization: Ying Bai and Bing Han Neurosci Bull.
Investigation: Ying Bai, Zhongqiu Zhou, and Bing Han https://doi.org/10.1007/s12264-022-00950-6

Volume 1 Issue 3 (2022) 8 https://doi.org/10.36922/an.v1i3.278