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Advanced Neurology

ORIGINAL RESEARCH ARTICLE
Transient receptor potential melastatin 7

signaling in U251 cell migration and invasion
involves calcineurin

1
2
Haifan Gong , Raymond Wong , Julia Bandura , James T. Rutka ,
1
1
Zhong-Ping Feng *, and Hong-Shuo Sun 1,2,3,4*
1
1 Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario,
Canada
2 Department of Surgery, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
3 Department of Pharmacology and Toxicology, Temerty Faculty of Medicine, University of Toronto,
Toronto, Ontario, Canada
4 Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada

Abstract

Transient receptor potential melastatin 7 (TRPM7) is a divalent cation channel that
has crucial functions in glioblastoma (GBM), which remains the most prevalent and
lethal primary brain tumor in adults. Altering TRPM7 activity has previously been
reported to affect GBM cell function (i.e., migration, invasion, and proliferation),
thus elucidating the TRPM7-mediated signaling pathway in GBM could reveal novel
therapeutic targets. Calcineurin, a Ca -dependent phosphatase, also influences GBM
2+
*Corresponding authors: cell survival and migration. However, the role or the relationship between TRPM7 and
Zhong-Ping Feng calcineurin in GBM signaling has not previously been investigated. In this study, we
(zp.feng@utoronto.ca)
Hong-Shuo Sun provide evidence that there is a possible interaction between TRPM7 and calcineurin
(hss.sun@utoronto.ca) in the GBM cell line U251. Moreover, we employed pharmacological approaches to
Citation: Gong H, Wong R, show that TRPM7 regulates calcineurin function, thereby suggesting that calcineurin
Bandura J, et al., 2023, Transient is a potential downstream target of TRPM7 signaling in U251 cell migration and
receptor potential melastatin 7 invasion.
signaling in U251 cell migration and
invasion involves calcineurin.
Adv Neuro, 2(3): 334.
https://doi.org/10.36922/an.334 Keywords: Glioblastoma; Transient receptor potential melastatin 7 channels; Calcineurin
Received: January 19, 2023
Accepted: July 10, 2023
Published Online: July 24, 2023 1. Introduction
Copyright: © 2023 Author(s). The ubiquitously expressed transient receptor potential melastatin 7 (TRPM7) channel
This is an Open-Access article is involved in the cellular homeostasis of divalent ions [1-3] . Containing both an ion
distributed under the terms of the channel and a kinase domain [4-6] , TRPM7 plays a critical role in cancer cell function, and
Creative Commons Attribution
License, permitting distribution, inhibition of overexpressed TRPM7 has been identified as a potential pharmaceutical
and reproduction in any medium, intervention [7-11] .
provided the original work is
properly cited. Glioblastoma (GBM) is the most lethal malignant primary brain tumor in adults, with
Publisher’s Note: AccScience an estimated 5-year survival rate of 5.1% [12,13] . Current treatment approaches, including
Publishing remains neutral with surgical resection, radiotherapy, and chemotherapy, are untargeted, resulting in suboptimal
regard to jurisdictional claims in [14]
published maps and institutional outcomes . Thus, novel treatment options are urgently required. Over the past decade,
affiliations. there has been growing evidence demonstrating the critical role of TRPM7 in regulating

Volume 2 Issue 3 (2023) 1 https://doi.org/10.36922/an.334