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Advanced Neurology TG100-115 suppresses glioblastoma cell functions

A

B

Figure 4. TG100-115 inhibited the migration of U251 cells at different doses for 24 h. (A) Representative images of wound healing. After being scratched
with a 200 µL pipette tip, U251 cells were treated with TG100-115 at doses of 60, 120, 160 µM or 0.1% DMSO. Then, the images were captured at
24 h, and the gap closure was analyzed. Scale bar: 150 µm, magnification: 10×. (B) The wound closure of TG100-115 treatment at different doses was
significantly decreased compared with DMSO.
Note: Statistical significance determined at **p<0.01 and ***p<0.001 using one-way analysis of variance with subsequent Newman–Keuls test, n ≥ 6.
Abbreviation: DMSO: Dimethyl sulfoxide.

light on its potential role in cellular processes associated 115 significantly reduced the viability and proliferation of
with migration and invasion. U251 cells in a dose-dependent manner, with an IC of
50
155.2 µM. In addition, TG100-115 exhibited substantial
3.7. TG100-115 did not affect the protein expression inhibitory effects on U251 cell migration and invasion.
of TRPM7 Furthermore, we found that the anti-GBM activities
Finally, we investigated the impact of TG100-115 treatment of TG100-115 were involved in PI3K/Akt and cofilin-
on the protein expression of TRPM7 in U251 cells. Following dependent signaling. In this study, experiments were
a 24-h exposure to either DMSO (0.1%) or TG100-115 (150 initially performed using both U87 and U251 glioma cell
µM), we assessed TRPM7 expression through western lines, with preliminary data obtained from both models.
immunoblotting. As shown in Figure S2, the raw blot However, due to variability in experimental conditions
images are provided in Figure S16, TG100-115 exhibited no and inconsistencies in U87 cell status, only the U251 cell
significant influence on the protein expression of TRPM7 data have been reported here. Nevertheless, the relevant
when compared to the control group (19.1 ± 3.8% vs. 20.3 ± results from U87 cells are included in Figure S3, raw blot
4.6%, respectively, p>0.05, n = 4). This suggests that TG100- images are provided in Figure S17. This data demonstrated
115 did not markedly influence the protein expression of that TG100-115 significantly reduced U87 cell viability,
TRPM7 under the conditions examined in U251 cells. migration, colony formation, and Akt phosphorylation,
consistent with the findings observed in U251 cells. Thus,
4. Discussion the results from both cell lines support the conclusion

This study demonstrates the role of TG100-115 in GBM that TG100-115 exerts anti-GBM effects via similar
cell biological functions. Our results showed that TG100- mechanisms.

Volume 4 Issue 3 (2025) 94 doi: 10.36922/AN025110023