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Gene & Protein in Disease Pyroptosis-related LncRNAs in pediatric AML

A B

Figure 10. Representative results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. (A) GO and (B) KEGG.

Immune checkpoints are molecules that are expressed as a risk factor of AML with poorer prognosis [32,33] . In other
on immune cells and can regulate the immune process, studies, TRAF3IP2-AS1 has also been found to be related
thus playing an important role in immune effects . The to N -methyladenosine and ferroptosis, affecting the
[28]
6
outcomes showed that there were significant differences in prognosis and treatment of patients [34,35] . SNHG29, which
the expression of CTLA-4, PD-1, and PD-L1, which have was identified as a protective factor in our study, has been
been proven as essential immune checkpoints in pediatric found in previous studies that it inhibits the ubiquitination
AML, between clusters 1 and 3, with higher expressions in degradation of yes-associated protein (YAP) by binding
the poorer prognostic cluster (cluster 1); the results were to it, thus promoting the expression of downstream target
similar to Jiang’s study [29,30] . Interestingly, we also found gene PD-L1 and subsequently anti-AML immunity [36,37] .
that LAG3 had the same expression difference in these Besides, in Han’s study, they found that SNHG29 has a
clusters. role in carcinogenesis through the miR-223-3p/CTNND1
[38]
Using LASSO-Cox and multi-Cox, we confirmed axis . Besides, Li has found that SNHG29 can indirectly
that seven out of 841 DE-lncRNAs are deserving of affect the expression of BAALC, a gene upregulated in
inclusion in the construction of a prognostic signature AML, by sponging miR-380-3p and negatively modulating
for predicting OS in pediatric AML, in which the miR-380-3p expression as a competing endogenous RNA
[39]
7 PPR-lncRNAs are TRAF3IP2-AS1, AL157871.6, (ceRNA) . The expression of ASB16-AS1 was positively
SNHG29, ASB16-AS1, AC007216.3, AP001318.1, and associated with risk score in our study. In another study,
AC127496.5. TRAF3IP2-AS1, which was lowly expressed ASB16-AS1 affected more than ten immune-related signal
in the high-risk group, has been found to play a key role pathways in multiple cancer types and played a key role
in the etiopathogenesis of various autoimmune diseases in the recruitment and functional regulation of tumor-
by negatively regulating human IL-17 signaling through infiltrating immune cells . In AML therapeutic area,
[40]
[31]
the downregulation of activator 1 (Act1) expression . NF-kappa B (NF-κB) pathway has been regarded as a target.
IL-17 is a pro-inflammatory cytokine that is secreted by In a study conducted by Bosman et al., they discovered
activated CD4 T-cells, involved in inducing and mediating that the performance of transforming growth factor-β
proinflammatory responses, and increasingly recognized activated kinase 1 (TAK1) is related to the overexpression

Volume 2 Issue 1 (2023) 13 https://doi.org/10.36922/gpd.v2i1.230