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Gene & Protein in Disease m1A-mediated ESCCAL-1 promotes ESCA stemness

A B

C

D

Figure 5. The role of ALKBH3/ESCCAL-1 axis in regulating ESCA stemness. (A) Western blot was applied to examine the protein levels of CD44 and KLF4
in ESCA cells following ALKBH3 silence alone or combined with ESCCAL-1 overexpression, n = 3. (B) A colony formation experiment was carried out to
observe the role of the ALKBH3/ESCCAL-1 axis in ESCA growth, n = 3. **P < 0.01. TE1 cells were transfected with si-ALKBH3 or si-ALKBH3 + OE-AL1.
(C) Tumor sphere formation assay depicted that overexpression of ESCCAL-1 rescued the effects of ALKBH3 silence on ESCA stemness. *P < 0.05. Scale
bar = 100 μm. TE1 cells were transfected with si-ALKBH3 or si-ALKBH3 + OE-AL1. (D) A mechanistic model of ALKBH3-mediated ESCCAL-1 in ESCA
stemness, n = 3.
ESCCAL-1: Esophageal squamous cell carcinoma associated long non-coding RNA 1; ESCA: Esophageal cancer.

Asian tumor samples was significantly higher than that in tumors, such as prostate cancer [26,27] . Moreover, the
Caucasian patients. According to Global Cancer Statistics, activation of this signal can be regulated by upstream
the incidence of male ESCA patients is two to three folds lncRNAs [26,27] . A recent study has found that the androgen
higher than that of female patients, and the Asian region receptor functions as an oncogenic promoter in ESCA .
[28]
shows a higher incidence of ESCA compared with Western Therefore, ESCCAL-1 might contribute to the difference in
countries and regions . Therefore, we speculate that ESCA incidence between males and females by regulating
[1]
ESCCAL-1 might be one of the key factors leading to the the androgen receptor signaling. However, the relationship
male ESCA and Asian ESCA. Androgen receptor signaling between ESCCAL-1 and the gender or ethnicity of ESCA
is widely considered a risk factor for male genitourinary patients needs to be further confirmed.

Volume 2 Issue 1 (2023) 8 https://doi.org/10.36922/gpd.305