Page 31 - GTM-1-2

Global Translational Medicine

ORIGINAL RESEARCH ARTICLE
Comparative proteomic analysis of hearts from

mice with high-fat diet-induced metabolic
cardiomyopathy

Zongzhe Jiang , Mingyang Pang , and Wei Huang 2,3,4 *
2†
1†
1 Luzhou Key Laboratory of Cardiovascular and Metabolic Diseases, Affiliated Hospital of Southwest
Medical University, Luzhou, Sichuan, China
2 Department of Cardiology, Chinese PLA Central War Command General Hospital, Wuhan, 430 010,
China
3 Division of Cardiology, Department of Internal Medicine, and Hubei Key Laboratory of Genetics
and Molecular Mechanisms of Cardiological Disorders, Tongji Hospital, Tongji Medical College,
Huazhong University of Science and Technology, Wuhan, 430 010, China
4 Gene Therapy Center and the Institute of Hypertension, Huazhong University of Science and
Technology, Wuhan, 430 010, China

Abstract

In patients with obesity or type 2 diabetes, the accumulation of lipotoxic
by-products in cardiomyocytes leads to apoptosis and contractile dysfunction,
eventually resulting in metabolic cardiomyopathy (MC). However, the underlying
mechanisms remain unclear. Inb this study, a comparative proteome analysis was
† These authors contributed equally conducted to evaluate the differentially expressed proteins (DEPs) in the hearts of
to this work. normal mice on standard diet (control group) and of high-fat diet (HFD)-induced
*Corresponding author: MC mice (HFD group). We identified 90 DEPs unique to the control group and 18
Wei Huang DEPs unique to the HFD group. In 90 DEPs unique to the control group, only 74
(huangwei0521@126.com) DEPs were annotated in the gene ontology (GO) database. These annotated DEPs
Citation: Jiang Z, Pang M, and are involved in 114 biological processes, 68 molecular functions, and 174 cellular
Huang W, 2022, Comparative components. In 18 DEPs unique to the HFD group, only 14 DEPs were annotated in
proteomic analysis of hearts from the GO database. These annotated DEPs are involved in 24 biological processes, 22
mice with high-fat diet-induced
metabolic cardiomyopathy. Global molecular functions, and six cellular components. Protein levels of two fatty acid
Transl Med, 1(2): 137. metabolism-related enzymes, carnitine palmitoyltransferase 1B (CPT1B) and acetyl-
https://doi.org/10.36922/gtm.v1i2.137 CoA acyltransferase 2 (ACAA2), in the hearts of the mice in control group and HFD
Received: June 17, 2022 group were analyzed by immunostaining and Western blot. The results showed that
Accepted: August 12, 2022 the protein levels of CPT1B and ACAA2 were elevated in hearts of the mice in HFD
Published Online: September 15,
2022 group, which were consistent with the proteomic analysis. Our results reveal the
differentially expressed proteome related to the progression of MC, providing a
Copyright: © 2022 Author(s).
This is an Open Access article series of potential therapeutic targets for MC.
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution, Keywords: High-fat diet; Metabolic cardiomyopathy; Proteome analysis
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience 1. Introduction
Publishing remains neutral with
regard to jurisdictional claims in According to the report of The International Diabetes Federation, about 500 million
published maps and institutional people will become obese and insulin-resistant, while more than 500 million people
affiliations.

Volume 1 Issue 2 (2022) 1 https://doi.org/10.36922/gtm.v1i2.137