Page 84 - GTM-4-1

Global Translational Medicine Gelatin-based cell carriers for tooth-germ organoids

A

B

Figure 4. Osteogenic induction of human dental pulp stem cells (hDPSCs) on/in different carriers. hDPSCs cultured on/in different carriers for 10 days,
followed by incubation in osteogenic induction medium (upper row) or growth medium (lower row) for an additional 10 days. Samples were stained with
alizarin red solution and subjected to extensive washes. (A) Representative images of GelMA MS (i and v), GelMA MP (ii and vi), Gel MS (iii and vii),
and GelMA HG (iv and viii) are shown. Representative calcium deposits are indicated by white arrows. Scale bar=100 µm; magnification power = 100×.
(B) Staining was semi-quantitatively measured using ImageJ. Data are presented as mean ± SD (n > 15; *P < 0.05, ***P < 0.005).
Abbreviations: AU: Arbitrary units; GelMA HG: Methacrylate gelatin hydrogel; GelMA MP: Methacrylate gelatin microparticles; GelMA MS: Methacrylate
gelatin microspheres; Gel MS: Gelatin microspheres.

predominantly occurred between the particles. Increased constructs in GelMA MS, GelMA MP, and Gel MS may
cell-cell communication and cell density have been offer advantages in tooth organoid generation compared
shown to promote osteogenic differentiation of hDPSC. to the surface-limited deposits observed in GelMS HG,
37
The distribution of calcium deposits throughout the potentially leading to improved distribution of deposited

Volume 4 Issue 1 (2025) 76 doi: 10.36922/gtm.5897