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International Journal of Bioprinting                                     Design of dual-unit porous scaffold
























            Figure 4. Setting of boundary conditions for finite element simulation of
            compression.

            3. Results and discussion

            3.1. Macroscopic morphology analysis
            Figure 5 shows a camera photo of the continuous
            transition  connected  scaffolds,  which  exhibit  consistent
            diameters and heights with the designed scaffold models.
            The top view reveals seamless connections between the
            inner and outer layers of the porous scaffold, indicating
            excellent continuity. Moreover, no visible deformations
            or  pore  plugging  phenomena  were  observed  on  the   Figure 5. The macro-morphologies of the designed porous scaffold.
            scaffold surfaces, confirming successful preparation of
            continuous transition connected scaffolds. A slight sticky
            powder phenomenon was observed on the scaffold surface,   the designed ones because of the gradual decrease in the
            primarily arising  from  the thermal  gradient  between   volume of powder particles on the surface of the scaffolds
            successive layers during the SLM preparation process.   and the increase in the amount of powder shedding during
            This condition caused the adherence of a small amount of   the melting process.  Based on these previously reported
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            powder to the scaffold, giving it a sticky appearance. Since   observations, we confirmed that dual-unit continuous
            the amount of adhered powder was minute, the effect on   transition porous scaffold was successfully designed and
            the mechanical properties of the scaffold was very minimal.   prepared in this work.
            Figure  6  shows  the  micro-CT  scanning  reconstruction   Figure 7 shows the SEM images of the prepared porous
            images of the scaffolds, which is consistent with the profile   scaffolds. It can be seen from Figure 7A, D, F, G, and I
            of the porous scaffold depicted in the Figure 5. Detailed   that the porous scaffold is covered with a small amount of
            micro-CT images show that the connection between the   sticky powder, and there are a large number of “dendrites”
            two units of the scaffold is smooth and continuous, and   at the unit joints of the P combination structure, resulting
            there is no deformation and blockage.              in a smaller aperture of the P unit as compared to the
               The porosities of the scaffolds measured by the   designed one. Figure 7E and H depicts the presence of
            Archimedes drainage method are presented in Table 4. It   unconnected areas in the G-D and D-G porous scaffolds,
            can be seen that the porosities of the porous scaffold are   which  can  be  attributed  to  the  fusion  penetration
            basically consistent with the designed porosity, and the   phenomenon during the SLM process. This phenomenon
            error is within 4%, which is within the permissible range of   is caused by the support thickness being smaller than the
            sample preparation error. The measured porosity of a small   design size, which impacts the mechanical properties
            number of samples is smaller than the designed models,   of the scaffold to some extent. Thus, it is essential to
            because the actual pore size of the sample is smaller than   consider the accuracy of processing and the minimum
            the design value due to the surface sticky powder and   design size while designing the scaffold. 43,44  While sound
            the “step effect” during SLM preparation.  However, the   continuous transition connections were formed in the
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            porosity of most samples is slightly larger than that of   P-G, G-P, P-D, and D-P porous scaffolds (Figure 7D, F, G,

            Volume 10 Issue 1 (2024)                       374                          https://doi.org/10.36922/ijb.1263
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