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International Journal of Bioprinting TPMS bone scaffold

Figure 6. The effects of different scaffold composites on inducing osteogenesis and angiogenesis in vitro. (A) ALP staining of MC3T3 cells cultured in the
scaffold conditioned medium for 14 days. (B) Alizarin red staining of MC3T3 cells cultured in the scaffold-conditioned medium for 21 days. (C) Brightfield
mages of HUVEC tube formation induced by different scaffold medium extracts. (D) Number of calcium nodules in Alizarin red staining experiments.
(E) Quantitative analysis of the vascular meshes in angiogenic experiments. Data were analyzed using one-way ANOVA, n ≥ 3. *P < 0.05, **P < 0.01, ***P
< 0.001, ****P < 0.0001. Notes: IT, TPMS scaffold loaded with I-PRF; NC, negative control; SIT, TPMS scaffold loaded with I-PRF and SDF-1; ST, TPMS
scaffold loaded with SDF-1; T, TPMS scaffold.

good biocompatibility, facilitating cellular migration, osteoblasts on the scaffold, enhancing its osteogenic
proliferation, and osteogenesis, consistent with our activity and accelerating the formation of new dense
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findings in vitro. Moreover, the addition of I-PRF and bone tissue. Our findings showed that the addition of
SDF-1 has been reported to augment the behavior of I-PRF or SDF-1 alone or in combination substantially

Volume 10 Issue 1 (2024) 470 https://doi.org/10.36922/ijb.0153