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International Journal of Bioprinting Microfluidic-assisted 3D bioprinting

Figure 6.Types of 3D bioprinting extruders. (a) In DIW approaches, a syringe is used to extrude a biomaterial or a bioink directly onto the substrate. (b)
Coaxial needles and glass capillaries are used in coaxial wet-spinning bioprinting to quickly solidify a material precursor with a crosslinking sheath solution
coming from the outer shell. (c) Upstream the coaxial needle, the integration of a microfluidic chip enables the creation of more complex patterns within
the fiber. The microfluidic printhead can be provided with (i) a metallic connector or a glass capillary (c3DMB) or with (ii) a PDMS nozzle (a3DMB).

Table 2. Diverse approaches for 3D biofabrication of functional fiber-based 3D in vitro models
Bioprinting Biomaterial Crosslinker Fiber type Cell type Application Ref.
approach
Coaxial Alginate/GelMA/PEG- CaCl , Irgacure Hollow VSMCs Vasculature 206
2
wet-spinning DA, Pluronic F127 2959
3D bioprinting GelMA, chondroitin CaCl , Irgacure Solid hBM-MSCs Cartilage 32
sulfate amino ethyl 2959 2
methacrylate (CS-AE-
MA), hyaluronic acid
methacrylate (HAMA)
GelMA, alginate, Irgacure 2959 Hollow multi-layered C2C12, NIH-3T3, Urothelial tissue, 121
PEGOA HUCs, BdSMCs, Vasculature
hSMC, HUVECs
Alginate, chitosan CaCl , EDC, Solid HepaRG Hepatic tissue 162
2
Genipin
Alginate, Matrigel CaCl Solid iPSCs Neural tissue 167
2
Alginate, GelMA, PVA CaCl Solid, core-shell, multi-core, / Multi-functional 168
2
multi-layered, Janus, spin- and tailorable
dle-knotted, structured fibers
Alginate CaCl , CaCl 2 Hollow L929 Nutrient delivery 169
2
bath
Continued

Volume 10 Issue 1 (2024) 59 https://doi.org/10.36922/ijb.1404