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International Journal of Bioprinting                                          Optimizing inkjet bioprinting




            that affects the cell viability and cell phenotype. Next, we   References
            provide in-depth discussion on the droplet formation
            process, dynamics of both polymer-based and cell-laden   1.   Ng WL, Chua CK, Shen Y-F. Print me an organ! Why we are
            droplet impact on media. This discussion underscores   not there yet. Prog Polym. Sci. 2019;97:101145.
            their pivotal role in achieving successful cell deposition.      doi: 10.1016/j.progpolymsci.2019.101145
            Furthermore, we emphasize the critical significance of   2.   Sun W, Starly B, Daly AC, et al. The bioprinting roadmap.
            3D  hydrogel  matrices,  elucidating  their  critical  role  in   Biofabrication. 2020;12(2):022002.
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            cells to adhere, proliferate, and differentiate. Lastly, we   3.   Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J,
            take a glimpse into the promising future of inkjet-based   et al. From shape to function: The next step in bioprinting.
            bioprinting, particularly in the fields of skin and lung tissue   Adv Mater. 2020;32(12):1906423.
            engineering. We also consider the potential integration of      doi: 10.1002/adma.201906423
            machine learning to enhance the precision and efficiency   4.   Gudapati H, Dey M, Ozbolat I. A comprehensive review
            of inkjet-based bioprinting and the development of    on droplet-based bioprinting: past, present and future.
            complementary bioprinting technologies to  fabricate   Biomaterials. Biomaterials. 2016;102:20-42.
            larger tissue constructs with increased complexities in a      doi: 10.1016/j.biomaterials.2016.06.012
            scalable manner. In summary, our comprehensive review   5.   Ng WL, Yeong WY, Naing MW. Microvalve bioprinting of
            has unveiled  the multifaceted aspects of inkjet-based   cellular droplets with high resolution and consistency. Proc
            bioprinting, emphasizing its potential to revolutionize   Int Conf Prog Addit Manuf. 2016;397-402.
            tissue engineering and regenerative medicine while also      doi: 10.3850/2424-8967_V02-236
            paving  the way  for advanced,  machine  learning-driven   6.   Ng  WL,  Lee JM,  Yeong  WY,  Naing  MW.  Microvalve-
            optimization of the cell deposition process.          based bioprinting – process, bio-inks and applications.
                                                                  Biomaterials. Sci. 2017;5(4):632-647.
            Acknowledgments                                       doi: 10.1039/C6BM00861E
            Wei Long Ng would like to acknowledge support from   7.   Koch L, Deiwick A, Franke A,  et al. Laser bioprinting
            NTU Presidential Postdoctoral Fellowship.             of  human  induced  pluripotent  stem  cells—the  effect  of
                                                                  printing and biomaterials on cell survival, pluripotency, and
            Funding                                               differentiation. Biofabrication. 2018;10(3):035005, 1-21.
                                                                  doi: 10.1088/1758-5090/aab981
            This work is supported and funded by NTU Presidential   8.   Kotlarz M, Ferreira AM, Gentile P, Russell SJ, Dalgarno K.
            Postdoctoral Fellowship and HP Inc.
                                                                  Droplet-based bioprinting enables the fabrication of cell–
                                                                  hydrogel–microfibre composite tissue precursors.  Bio-Des
            Conflict of interest                                  Manuf. 2022;5(3):512-528.
            Viktor Shkolnikov is an employee of HP Inc. and may own      doi: 10.1007/s42242-022-00192-5
            stock as part of the standard compensation package.  9.   Ng WL, Yeong WY, Naing MW. Potential of bioprinted films
                                                                  for skin tissue engineering. Proceedings of the 1st International
            Author contributions                                  Conference on Progress in Additive Manufacturing. 2014;441-
                                                                  446.
            Conceptualization: Wei Long Ng, Viktor Shkolnikov      doi: 10.3850/978-981-09-0446-3_065
            Visualization: Wei Long Ng, Viktor Shkolnikov
            Writing – original draft: Wei Long Ng, Viktor Shkolnikov   10.  Ozbolat IT, Hospodiuk M. Current advances and future
                                                                  perspectives in extrusion-based bioprinting.  Biomaterials.
            Writing – review & editing: Wei Long Ng, Viktor Shkolnikov
                                                                  2016;76:321-343.
                                                                  doi: 10.1016/j.biomaterials.2015.10.076
            Ethics approval and consent to participate
                                                               11.  Ng WL, Yeong WY, Naing MW. Development of
            Not applicable.                                       polyelectrolyte chitosan-gelatin hydrogels for skin
                                                                  bioprinting. Procedia CIRP. 2016; 49:105-112.
            Consent for publication                               doi: 10.1016/j.procir.2015.09.002

            Not applicable.                                    12.  Zhuang P, Ng WL, An J, Kai Chua C, Tan LP. Layer-by-layer
                                                                  ultraviolet assisted extrusion-based (UAE) bioprinting of
            Availability of data                                  hydrogel constructs with high aspect ratio for soft tissue
                                                                  engineering applications. PLoS One. 2019;14(6):e0216776.
            Not applicable.                                       doi: 10.1371/journal.pone.0216776


            Volume 10 Issue 2 (2024)                       201                                doi: 10.36922/ijb.2135
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