Page 209 - IJB-10-2
P. 209
International Journal of Bioprinting Optimizing inkjet bioprinting
that affects the cell viability and cell phenotype. Next, we References
provide in-depth discussion on the droplet formation
process, dynamics of both polymer-based and cell-laden 1. Ng WL, Chua CK, Shen Y-F. Print me an organ! Why we are
droplet impact on media. This discussion underscores not there yet. Prog Polym. Sci. 2019;97:101145.
their pivotal role in achieving successful cell deposition. doi: 10.1016/j.progpolymsci.2019.101145
Furthermore, we emphasize the critical significance of 2. Sun W, Starly B, Daly AC, et al. The bioprinting roadmap.
3D hydrogel matrices, elucidating their critical role in Biofabrication. 2020;12(2):022002.
providing a conducive microenvironment for printed doi: 10.1088/1758-5090/ab5158
cells to adhere, proliferate, and differentiate. Lastly, we 3. Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J,
take a glimpse into the promising future of inkjet-based et al. From shape to function: The next step in bioprinting.
bioprinting, particularly in the fields of skin and lung tissue Adv Mater. 2020;32(12):1906423.
engineering. We also consider the potential integration of doi: 10.1002/adma.201906423
machine learning to enhance the precision and efficiency 4. Gudapati H, Dey M, Ozbolat I. A comprehensive review
of inkjet-based bioprinting and the development of on droplet-based bioprinting: past, present and future.
complementary bioprinting technologies to fabricate Biomaterials. Biomaterials. 2016;102:20-42.
larger tissue constructs with increased complexities in a doi: 10.1016/j.biomaterials.2016.06.012
scalable manner. In summary, our comprehensive review 5. Ng WL, Yeong WY, Naing MW. Microvalve bioprinting of
has unveiled the multifaceted aspects of inkjet-based cellular droplets with high resolution and consistency. Proc
bioprinting, emphasizing its potential to revolutionize Int Conf Prog Addit Manuf. 2016;397-402.
tissue engineering and regenerative medicine while also doi: 10.3850/2424-8967_V02-236
paving the way for advanced, machine learning-driven 6. Ng WL, Lee JM, Yeong WY, Naing MW. Microvalve-
optimization of the cell deposition process. based bioprinting – process, bio-inks and applications.
Biomaterials. Sci. 2017;5(4):632-647.
Acknowledgments doi: 10.1039/C6BM00861E
Wei Long Ng would like to acknowledge support from 7. Koch L, Deiwick A, Franke A, et al. Laser bioprinting
NTU Presidential Postdoctoral Fellowship. of human induced pluripotent stem cells—the effect of
printing and biomaterials on cell survival, pluripotency, and
Funding differentiation. Biofabrication. 2018;10(3):035005, 1-21.
doi: 10.1088/1758-5090/aab981
This work is supported and funded by NTU Presidential 8. Kotlarz M, Ferreira AM, Gentile P, Russell SJ, Dalgarno K.
Postdoctoral Fellowship and HP Inc.
Droplet-based bioprinting enables the fabrication of cell–
hydrogel–microfibre composite tissue precursors. Bio-Des
Conflict of interest Manuf. 2022;5(3):512-528.
Viktor Shkolnikov is an employee of HP Inc. and may own doi: 10.1007/s42242-022-00192-5
stock as part of the standard compensation package. 9. Ng WL, Yeong WY, Naing MW. Potential of bioprinted films
for skin tissue engineering. Proceedings of the 1st International
Author contributions Conference on Progress in Additive Manufacturing. 2014;441-
446.
Conceptualization: Wei Long Ng, Viktor Shkolnikov doi: 10.3850/978-981-09-0446-3_065
Visualization: Wei Long Ng, Viktor Shkolnikov
Writing – original draft: Wei Long Ng, Viktor Shkolnikov 10. Ozbolat IT, Hospodiuk M. Current advances and future
perspectives in extrusion-based bioprinting. Biomaterials.
Writing – review & editing: Wei Long Ng, Viktor Shkolnikov
2016;76:321-343.
doi: 10.1016/j.biomaterials.2015.10.076
Ethics approval and consent to participate
11. Ng WL, Yeong WY, Naing MW. Development of
Not applicable. polyelectrolyte chitosan-gelatin hydrogels for skin
bioprinting. Procedia CIRP. 2016; 49:105-112.
Consent for publication doi: 10.1016/j.procir.2015.09.002
Not applicable. 12. Zhuang P, Ng WL, An J, Kai Chua C, Tan LP. Layer-by-layer
ultraviolet assisted extrusion-based (UAE) bioprinting of
Availability of data hydrogel constructs with high aspect ratio for soft tissue
engineering applications. PLoS One. 2019;14(6):e0216776.
Not applicable. doi: 10.1371/journal.pone.0216776
Volume 10 Issue 2 (2024) 201 doi: 10.36922/ijb.2135

