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International Journal of Bioprinting Optimizing inkjet bioprinting

Figure 8. (A) Fabrication of 3D alveolar barrier models using type I alveolar cells (NCI-H1703), type II alveolar cells (NCI-H441), lung endothelial cells
(HULEC-5a), and lung fibroblasts (MRC5). Scale bar: 50 µm. (B) Schematic diagram of inkjet bioprinting process for fabrication of 3D alveolar barrier
model in a layer-by-layer manner. (C) Comparison of representative alveolar gene expression profiles between conventional and 3D inkjet-bioprinted
structured models. Relative mRNA expression was measured via qPCR for 2D cell culture, 3D non-structured, and 3D-structured models using specific
markers involved in (i–iii) intercellular junction formation, (iv–vi) epithelial ion channels and ion transport, and (vii–ix) pulmonary surfactant secretion.
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Each expression levels were normalized by the level of 2D cell culture models. Adapted from ref.

Volume 10 Issue 2 (2024) 199 doi: 10.36922/ijb.2135