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International Journal of Bioprinting Kidney hydrogel print for renal cancer model

Figure 5. Transcriptional profiles for ACHN cells in GelMA-based samples. (A) Volcano plot; (B) GO terms enriched in ACHN cells; (C) KEGG terms
enriched in ACHN cells.
4. Discussion Here, 10% GelMA was mixed with various
concentrations of kidney dECM powders, and the
3D cell culture models have been widely used in RCC resulting samples were systematically analyzed. We found
research. At present, however, few models can closely that their material properties were significantly changed,
recapitulate cell–matrix interactions or be used for and their mechanical properties and stability were all
accurate drug assessment. In this study, we prepared a significantly improved following the addition of dECM
novel 3D cell culture substrate for RCC cells by mixing powder. The mechanical behavior of a hybrid hydrogel
porcine kidney-derived dECM powders with GelMA. mainly depends on the interactions between additives or
GelMA is a bioink commonly used in 3D bioprinting for fillers and gel matrix. In this study, the dECM powders
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tissue engineering since it maintains the desirable bioactive were found to disperse well and restrict the growth of
characteristics of collagen. Although collagen is a major microcracks in GelMA. This in turn led to increases in the
ECM component found in the RCC stroma, numerous compressive modulus and stability. Similar results have
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other ECM components also play significant roles affecting also been observed in GelMA mixed with tofu powders or
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RCC progression. Therefore, biochemical components of decellularized bone powders used for bone regeneration.
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GelMA were supplemented with kidney dECM powder in
this study to enrich treatments so as to better simulate the Next, treatments including GelMA mixed with dECM
TME of RCC and promote printability. powders were further used to culture ACHN cells to examine
Volume 10 Issue 2 (2024) 289 doi: 10.36922/ijb.1413