Page 269 - IJB-10-3

International

Journal of Bioprinting

RESEARCH ARTICLE
3D-bioprinted bone scaffolds incorporating SR1

nanoparticles enhance blood vessel regeneration
in rat calvarial defects

KyeongWoong Yang , Donghyun Lee , Kyoung Ho Lee , Woong Bi Jang , Hye
1,2
1
1
2
Ji Lim , Eun Ji Lee , Hojun Jeon , Donggu Kang , Gi Hoon Yang , KyeongHyeon
2
2
3
3
3
Lee , Yong-Il Shin , Sang-Cheol Han , SangHyun An *, and Sang-Mo Kwon *
6
1
2
4,5
4
1 Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation (K-MEDI Hub),
Dong-gu, Daegu, Republic of Korea
2 Laboratory of Regenerative Medicine and Stem Cell Biology, Department of Physiology, Medical
Research Institute, School of Medicine, Pusan National University, Yangsan, Republic of Korea
3
Research Institute of Additive Manufacturing and Regenerative Medicine, Baobab Healthcare Inc.,
Ansan, Gyeonggi-Do, Republic of Korea
4 Science of Convergence, School of Medicine, Pusan National University, Yangsan, Republic of Korea
5 Department of Rehabilitation Medicine, Pusan National University Yangsan Hospital, Yangsan,
Republic of Korea
6 CEN Co., Ltd. Nano-Convergence Center, Miryang, Republic of Korea
(This article belongs to the Special Issue: 3D Printing of Bioinspired Materials)
Abstract
The inherent limitations of bone grafting in the treatment of critical-sized bone
defects have led to a growing demand for bone repair implants. Three-dimensional
(3D) bioprinting has emerged as a promising manufacturing technique for implants,
*Corresponding authors: offering flexibility in their structural design and the use of applicable materials.
SangHyun An
(ash4235@kmedihub.re.kr) Although numerous 3D-bioprinted bone scaffolds have been developed to enhance
Sang-Mo Kwon osteogenesis, angiogenesis remains a challenge. Angiogenesis is crucial for successful
(smkwon323@pusan.ac.kr) bone healing because the process forms blood vessels to deliver essential nutrients
Citation: Yang K, Lee D, Lee KH, and oxygen. Endothelial progenitor cells (EPCs) play a pivotal role in the early stages of
et al. 3D-bioprinted bone scaffolds vascularization. These cells, capable of differentiating into endothelial cells (ECs), are
incorporating SR1 nanoparticles recruited from the bone marrow to the injured area during the healing process. CD34
+
enhance blood vessel regeneration
in rat calvarial defects. cells, a subset of EPCs, have gained attention because of their neovascularization
Int J Bioprint. 2024;2024;10(3):1931. potential and ability to contribute to bone regeneration. The incorporation of CD34
+
doi: 10.36922/ijb.1931 cell-enhancing factors into 3D-printed bone scaffolds may facilitate successful bone
+
Received: September 27, 2023 healing in critical defects. StemRegenin-1 (SR1), a molecule that promotes CD34 cell
Accepted: November 3, 2023 expansion, has shown promising results in increasing CD34 hematopoietic stem and
+
Published Online: January 19, 2024 progenitor cell populations. This study aimed to investigate the sustained release of
Copyright: © 2024 Author(s). This SR1 from a collagen-based scaffold integrated with mesoporous silica nanoparticles
is an Open Access article distribut- (MSNs) to promote angiogenesis and enhance bone healing. The sustained release
ed under the terms of the Creative
Commons Attribution License, per- of SR1 from the collagen scaffold is hypothesized to promote angiogenesis, thereby
mitting distribution, and reproduction facilitating bone repair. In vitro studies have demonstrated the angiogenic potential
in any medium, provided the original of SR1; however, further in vivo investigations are required to establish its clinical
work is properly cited.
efficacy. This study contributes to the development of novel therapies targeting
Publisher’s Note: AccScience CD34 cells and demonstrates the potential of SR1 as a promising agent for promoting
+
Publishing remains neutral with
regard to jurisdictional claims in angiogenesis and enhancing bone healing in critical defects.
published maps and institutional
affiliations. Keywords: 3D printing; Bone scaffold; Angiogenesis; Calvarial defect; Bone regeneration

Volume 10 Issue 3 (2024) 261 doi: 10.36922/ijb.1931