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International Journal of Bioprinting Multi-physical field control inkjet bioprinting

Figure 7. Printing test of MFCPIB method. (A) Schematic drawing and photograph of the polyhedral pyramid. (B) Schematic drawing and photograph
of the stereoscopic text “SIA.” (C) Schematic drawing and photograph of the ear-shaped 3D structure. (D) Cell-laden vessel-like structures. (E, F) LIVE-
DEAD staining of vessel-like structure with SMCs on day 1 (E) and day 7(F). (G) Cell viability of SMCs in different layers of the vessel on day 1 and day 7.
(H) Quantification of cell proliferation on day 1 and day 7 using the CCK-8 assay; ∗∗p < 0.01, ∗∗∗p < 0.001. (I) Fluorescence micrographs showing SMCs
spreading morphology within the GelMA hydrogels.

chamber temperature is uniform. The temperature does and not undergo gelation, a condition convenient for the
not decrease too much when the single bottom plate subsequent assembly and molding of microdroplets. We
cools, and thus the printing material will remain uniform used the MFCPIB method to print the text structure “SIA,”

Volume 10 Issue 3 (2024) 374 doi: 10.36922/ijb.2120