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International Journal of Bioprinting Multi-physical field control inkjet bioprinting

that the MFCPIB method caused slight damage to cells Funding acquisition: Run Li, Liming Zhang
and maintained a high survival rate of cells after printing. Investigation: Huixuan Zhu, Run Li
Thus, the MFCPIB method exhibits promising potential Methodology: Huixuan Zhu, Yuejing Zheng, Heran Wang
for the utilization in printing cell-laden thermosensitive Project administration: Kai Guo
biomaterial and the in vivo experiments in future. Resources: Huixuan Zhu, Xiongfei Zheng
Software: Song Li, Feiyang Gao
4. Conclusion Validation: Yuejing Zheng

In conclusion, we successfully developed, tested, and Writing – original draft: Huixuan Zhu
implemented an innovative MFCPIB method for creating Writing – review & editing: Lianqing Liu
highly bioactive tissue-like structures using 5% GelMA. We Ethics approval and consent to participate
also demonstrated the feasibility of using this approach with
other concentrations of GelMA. Our method enables precise Not applicable.
control of the formation and properties of microdroplets
through temperature and pressure regulation. With this Consent for publication
method, we have successfully printed various 3D structures, Not applicable.
including a cell-laden vascular structure with an aspect
ratio of 4.0, without compromising the viability of cells. The Availability of data
MFCPIB method provides a new approach for inkjet printing
of thermosensitive materials such as GelMA. This printing Data are available from the corresponding author upon
method has potential applications in biological research reasonable request.
and clinical medicine, particularly for creating tissues such
as esophageal tissues and skin with high bioactivity. While References
the current method is unable to print hollow structures,
we believe that future work involving multi-printheads 1. Mota C, Camarero-Espinosa S, Baker MB, Wieringa P,
and low-concentration GelMA as a sacrificial material will Moroni L. Bioprinting: from tissue and organ development
to in vitro models. Chem Rev. 2020;120:10547–10607.
contribute to the creation of more complex tissues and doi: 10.1021/acs.chemrev.9b00789
organs for utilization in clinical medicine.
2. Zhou L, Fu J, He Y. A review of 3D printing technologies for
Acknowledgments soft polymer materials. Adv Funct Mater. 2020;30:2000187.
doi: 10.1002/adfm.202000187
None.
3. Wang H, Guo K, Zhang L, et al. Valve-based consecutive
bioprinting method for multimaterial tissue-like constructs
Funding with controllable interfaces. Biofabrication. 2021;13:035001.
This study was partially supported by the National doi: 10.1088/1758-5090/abdb86
Key Research and Development Program of China 4. Guo K, Wang H, Li S, et al. Bioprinting of light-crosslinkable
(SQ2020YFB130100), the Youth Innovation Promotion neutral-dissolved collagen to build implantable
Association of the Chinese Academy of Sciences (2021200), connective tissue with programmable cellular orientation.
the Research Equipment Development Program of the Biofabrication. 2023;15:035007.
Chinese Academy of Sciences (YJKYYQ20190045), the doi: 10.1088/1758-5090/acc760
National Natural Science Foundation of China (52205319), 5. Blaeser A, Duarte Campos DF, Puster U, Richtering W,
CAS Project for Young Scientists in Basic Research (YSBR- Stevens MM, Fischer H. Controlling shear stress in 3D
041), and the Foundation of State Key Laboratory of bioprinting is a key factor to balance printing resolution and
Robotics (2021-Z01, 2022-Z04, 2023-Z01). stem cell integrity. Adv Healthc Mater. 2016;5:326-333.
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Conflict of interest 6. Masaeli E, Marquette C. Direct-write bioprinting approach
to construct multilayer cellular tissues. Front Bioeng
The authors declare no conflicts of interest.
Biotechnol. 2020;7:478.
doi: 10.3389/fbioe.2019.00478
Author contributions
7. Li X, Chen J, Liu B, Wang X, Ren D, Xu T. Inkjet printing for
Conceptualization: Huixuan Zhu, Xiongfei Zheng biofabrication. In: Ovsianikov A, Yoo J, Mironov V, eds. 3D
Data curation: Huixuan Zhu, Chuang Ji, Runyang Zhu Printing and Biofabrication. Cham: Springer; 2018: 283-301.
Formal analysis: Huixuan Zhu, Kai Guo doi: 10.1007/978-3-319-45444-3_26

Volume 10 Issue 3 (2024) 376 doi: 10.36922/ijb.2120