Page 413 - IJB-10-3

International Journal of Bioprinting In situ thermal monitoring in bioprinting

5. Conclusion Supervision: Bianca Maria Colosimo, Davide Moscatelli
Writing – original draft: Simone Giovanni Gugliandolo,
In conclusion, this study has introduced a novel approach Bianca Maria Colosimo
for monitoring bioprinted constructs using thermal Writing – review & editing: Bianca Maria Colosimo,
imaging, demonstrating its feasibility and effectiveness. The Davide Moscatelli
absence of previous instances employing thermographic
imaging for the geometric analysis of bioprinting Ethics approval and consent to participate
constructs emphasizes the innovative nature of this work.
The successful capture of thermal image sequences in real- Not applicable.
time using the employed thermographic imaging device
allowed for in situ assessment of construct’s geometrical Consent for publication
characteristics. Specifically, the method highlighted the Not applicable.
ability to qualitatively distinguish between different layers
based on their temperature disparities. Availability of data
Our method effectively overcomes limitations posed Data are available from the corresponding author upon
by visible light systems in discerning bioink layers, reasonable request.
particularly in transparent bioinks. The method’s capacity
to segment deposited bioink filaments in real time enables References
the measurement of filament width during the printing
process. Additionally, the technique permitted the isolation 1. Moroni L, Boland T, Burdick JA, et al. Biofabrication: a
and separate reconstruction of individual layer profiles, guide to technology and terminology. Trends Biotechnol.
addressing the challenge of layer superposition. 2018;36(4):384-402.
doi: 10.1016/j.tibtech.2017.10.015
In summary, the application of thermal imaging
technology to construct bioprinting represents an 2. Ng WL, Chua CK, Shen YF. Print me an organ! Why we are
innovative step toward enhancing process monitoring not there yet. Prog Polym Sci. 2019;97:101145.
and quality control, laying the foundation for further doi: 10.1016/j.progpolymsci.2019.101145
advancements in bioprinting technology and its 3. Santoni S, Gugliandolo SG, Sponchioni M, Moscatelli D,
industrial implementation. Colosimo BM. 3D bioprinting: current status and trends—a
guide to the literature and industrial practice. Bio-Des
Acknowledgments Manuf. 2021;5(1):14-42.
doi: 10.1007/S42242-021-00165-0
None.
4. Pereira FDAS, Parfenov V, Khesuani YD, Ovsianikov A,
Mironov V. Commercial 3D bioprinters. In: Ovsianikov
Funding A, Yoo J, Mironov V, eds. 3D Printing and Biofabrication.
This research was partially funded by the European Cham: Springer; 2018: 535-549.
Commission under the “HORIZON-CL4-2021-DIGITAL- doi: 10.1007/978-3-319-45444-3_12
EMERGING-01 project BioProS - Biointelligent 5. Jang J, Park JY, Gao G, Cho D-W. Biomaterials-based 3D cell
Production Sensor to Measure Viral Activity” (grant printing for next-generation therapeutics and diagnostics.
agreement no. 101070120, 2022–2026). Biomaterials. 2018;156:88-106.
doi: 10.1016/j.biomaterials.2017.11.030
Conflict of interest 6. Constante G, Apsite I, Alkhamis H, et al. 4D biofabrication
The authors declare no conflicts of interest. using a combination of 3D printing and melt-electrowriting
of shape-morphing polymers. ACS Appl Mater Interfaces.
2021;13(11):12767-12776.
Author contributions doi: 10.1021/acsami.0c18608

Conceptualization: Bianca Maria Colosimo, Davide 7. Caleffi JT, Aal MCE, Gallindo H de OM, et al. Magnetic 3D
Moscatelli, Simone Giovanni Gugliandolo cell culture: state of the art and current advances. Life Sci.
Data curation: Simone Giovanni Gugliandolo 2021;286:120028.
Formal analysis: Simone Giovanni Gugliandolo, doi: 10.1016/j.lfs.2021.120028
Egon Prioglio 8. Hu X, Zheng J, Hu Q, et al. Smart acoustic 3D cell
Investigation: Simone Giovanni Gugliandolo, Egon Prioglio construct assembly with high-resolution. Biofabrication.
Methodology: Bianca Maria Colosimo, Simone Giovanni 2022;14(4).
Gugliandolo, Egon Prioglio doi: 10.1088/1758-5090/ac7c90

Volume 10 Issue 3 (2024) 405 doi: 10.36922/ijb.2021