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Choudhury D, et al.
                                                        [16]
           generated through air bubbles or piezoelectric actuator .   that allows bioprinting of cells and hydrogels with a cell-
           Since the technique requires a low-viscous bioink for   level resolution (Figure 2C). The technique has shown
           successful deposition, an additional crosslinking step is   great potential towards the realisation of scaffold-free
                                                                            [17]
                                       [15]
           accompanied in most of the cases .                  3D cell systems . It could be classified into two broad
                                                               categories:
                                                                ●Laser-guided direct cell printing: Manipulating the
           1.3.3  Laser-assisted Bioprinting                   difference in refractive index of the cells and suspending
                                                               solution, the laser traps and guides these cells in the
           Laser-assisted bioprinting is an emerging technology   desired direction .
                                                                             [18]
             A































             B





























           Figure 1. Distribution of 3D bioprinting patents (Adapted from ): (a) The top 10 assignee institutions as per the total number of patent
                                                         [13]
           families from 1  Jan 2000 till 1  July 2016. Three institutions are tied for the tenth position; (B) The top 10 most countries in which
                                  st
                      st
           priority filing was done for the first patent of a patent family. Four countries are tied for the tenth position.
                                       International Journal of Bioprinting (2018)–Volume 4, Issue 2         3
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