Page 212 - IJB-10-4

International

Journal of Bioprinting

RESEARCH ARTICLE
Three-dimensional-printed scaffolds

functionalized with stem cell recruitment and cell
respiration regulation for diabetic bone defects

Ke Jiang 1,2† , Caiping Yan 1,2† , Pengrui Zhang , Yongfu Xiong , Weikang Zhao ,
1
3
4,5
Jiangtao He , Lu Chen , Hanfeng Yang , Dianming Jiang *, Wei Huang *,
3
3
3
1
2
and Yuling Li *
1
1 Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University,
Orthopedic Laboratory of Chongqing Medical University, Chongqing, China
2 Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University,
Chongqing, China
3
Department of Orthopedics, Laboratory of Biological Tissue Engineering and Digital Medicine,
Nanomedicine Innovation Research and Development Transformation Institute, Affiliated Hospital
of North Sichuan Medical College, Nanchong, Sichuan, China
4 Department of Hepatobiliary Surgery, Academician (Expert) Workstation, Affiliated Hospital of
North Sichuan Medical College, Nanchong, Sichuan, China
5
Pharmaceutical College, Guangxi Medical University, Nanning, Guangxi, China
(This article belongs to the Special Issue: Advancements in 3D Printing, Microfluidics, and their Integrated
Applications)
† These authors contributed equally
to this work.
*Corresponding authors:
Dianming Jiang Abstract
(201296@hospital.cqmu.edu.cn)
Wei Huang High-glucose microenvironment in diabetic patients is a source of damage to the
(huangw511@163.com) cellular mitochondrial respiratory chain (MRC), which results in the generation of
Yuling Li reactive oxygen species (ROS) and leads to mitochondrial dysfunction, cellular
(lyl1987@nsmc.edu.cn)
senescence, and enhanced apoptosis, eventually causing weakened cellular
Citation: Jiang K, Yan C, Zhang P, migration and differentiation as well as physical dysfunction. In patients with
et al. Three-dimensional-printed
scaffolds functionalized with stem diabetic bone defects, the high-glucose microenvironment induces intracellular
cell recruitment and cell respiration mitochondrial dysfunction and diminished migration and differentiation of bone
regulation for diabetic bone defects. marrow mesenchymal stem cells (BMSCs), leading to impaired bone regeneration.
Int J Bioprint. 2024;10(4):2379.
doi: 10.36922/ijb.2379 In this study, polycaprolactone (PCL) porous scaffolds were prepared by three-
dimensional (3D) printing. The EPLQLKM (E7) and SS31 peptides were modified
Received: December 6, 2023 onto the surface of PCL porous scaffolds by chemical bonding to construct a
Accepted: February 6, 2024
Published Online: March 14, 2024 3D-printed porous scaffold system (PCL@SS31@E7) capable of stem cell recruitment
and regulation of cellular MRC to treat diabetic bone defects. In vitro cellular
Copyright: © 2024 Author(s).
This is an Open Access article energy metabolism and molecular biology experiments demonstrated that the
distributed under the terms of the scaffold system could continuously release E7 and SS31 peptides to recruit BMSCs,
Creative Commons Attribution improve MRC function, reduce proton leakage, protect mitochondria, and promote
License, permitting distribution,
and reproduction in any medium, proliferation and osteogenic differentiation of BMSCs to regenerate bone tissue in a
provided the original work is high-glucose environment. In vivo experiments confirmed that the PCL@SS31@E7
properly cited. porous scaffold induced regeneration of normal bone tissue in the area of femoral
Publisher’s Note: AccScience condylar bone defects in diabetic rats. The 3D-printed porous scaffold constructed
Publishing remains neutral with in this study is a novel biomaterial with the functions of stem cell recruitment and
regard to jurisdictional claims in
published maps and institutional targeted regulation of MRC and provides a new direction for the treatment of various
affiliations. diseases related to diabetes and MRC dysfunction.

Volume 10 Issue 4 (2024) 204 doi: 10.36922/ijb.2379