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International Journal of Bioprinting 3D bioprinting of composite hydrogels

Figure 9. Cell morphology on GG (left) and GG–3PEI (right) 3D-bioprinted scaffolds after 5 days of culture. Abbreviations: GG, gellan gum; PEI,
polyethyleneimine. Scale bars: 20 µm (left); 200 µm (right).

3PEI bioink for ophthalmic delivery and corneal Author contributions
tissue regeneration. The bioprinted cornea had high
transparency, stability, integrity, and shape fidelity, and it Conceptualization: John P. Frampton, Ali Zamanian
Data curation: Armin Badri
also ensured the growth and proliferation of a monolayer Formal analysis: Negin Khoshnood, Armin Badri
of corneal fibroblasts. Corneal fibroblasts spread on the Funding acquisition: Ali Zamanian, John P. Frampton
surface of the 3D-printed scaffold due to suitable surface Investigation: Ali Zamanian, John P. Frampton,
chemistry, i.e., containing bioactive and functional Negin Khoshnood
amine groups. Successful clinical application of this Methodology: Negin Khoshnood, Armin Badri
system will require in vivo testing, such as investigating Project administration: Ali Zamanian
the relationship between scaffold degradation and tissue Resources: Ali Zamanian
repair in animal models of corneal injury. In addition to Supervision: Ali Zamanian, John P. Frampton
establishing the biocompatibility of this novel ophthalmic Validation: Ali Zamanian, John P. Frampton
material, our data suggest that the rather low viscosity Visualization: Negin Khoshnood, Armin Badri
and rapid gelation time of the BSP-loaded GG–3PEI Writing – original draft: Negin Khoshnood
3D-bioprinted corneal scaffold will enable controlled Writing – review & editing: Ali Zamanian, John P. Frampton
delivery of ocular drugs, such as anti-inflammatory
agents, to aid in corneal repair. Ethics approval and consent to participate

Acknowledgments Not applicable.
None. Consent for publication

Funding Not applicable.

This work was supported by funds from the Materials Availability of data
and Energy Research Center (MERC) (Grant No.
14023050). Data are available from the corresponding author upon
reasonable request.
Conflict of interest References

The authors declare that they have no known competing
financial interests or personal relationships that could have 1. Dhar S, Mishra SK, Joshi A, et al. Evolving trends in corneal
appeared to influence the work reported in this paper. surgery. Delhi J Ophthalmol. 2023;33(4):280-289.

Volume 10 Issue 4 (2024) 332 doi: 10.36922/ijb.3440