Page 497 - IJB-10-4

International Journal of Bioprinting Embedded bioprinting of cartilage

Furthermore, the bioprinted cartilage exhibited the ability Investigation: Yang Wu, Xue Yang, Shengli Mi, Dong
to secrete cartilage-associated proteins. Nyoung Heo
Methodology: Xue Yang, Tianying Yuan, Seung Yeon Lee,
5. Conclusion Minghao Qin, Sung Jun Min
Writing - Original Draft: Xue Yang, Tianying Yuan,
Herein, embedded bioprinting based on a granular support
Minghao Qin
bath was investigated numerically and experimentally, Writing - Review & Editing: Yang Wu, Xue Yang, Seung
and the optimal process window was validated through Yeon Lee, Shengli Mi, Dong Nyoung Heo
the bioprinting of a zonally stratified articular cartilage.
A simulation model of the extrusion process was Ethics approval and consent to participate
established, and the disturbance of the printing nozzle in
the support bath was analyzed. By integrating the results Not applicable
of the simulation and experiments, a process window
was created, and the optimal set of printing parameters Consent for publication
was determined. The articular cartilage construct that Not applicable.
mimicked the anatomical structure of native cartilage
at the macroscopic level was bioprinted, featuring a tri- Availability of data
layered arrangement with vertical fibers in the bottom
zone, tilted fibers in the middle zone, and horizontal fibers All data that support the findings of this study are included
in the superficial zone. Moreover, cells exhibited robust in the article
survival and proliferation within the bioprinted cartilage,
with an evenly distributed cytoskeletal morphology. In References
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This work was funded by the Guangdong Natural Science behavior among Chinese older adults: patterns and
Foundation (grant number 2023A1515012439), the predictive factors. Int J Environ Res Public Health. 2021;
National Natural Science Foundation of China (grant 18(6):2969.
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Foundation (the Stable Support Plan Program; grant 4. Harris JD, Siston RA, Pan X, et al. Autologous chondrocyte
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through the Korea Health Industry Development Institute doi: 10.2106/JBJS.J.00049
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Conflict of interest doi: 10.1038/nrrheum.2014.157

The authors declare no conflicts of interest. 6. Wu Y, Ayan B, Moncal KK, et al. Hybrid bioprinting of
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Author contributions free tissue strands as building blocks. Adv Healthc Mater.
2020;9(22):e2001657.
Conceptualization: Yang Wu, Shengli Mi, Dong doi: 10.1002/adhm.202001657
Nyoung Heo 7. Mardones R, Jofré CM, Minguell JJ. Cell therapy and
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Guo, Jiarui Xie doi: 10.15283/ijsc.2015.8.1.48

Volume 10 Issue 4 (2024) 489 doi: 10.36922/ijb.3520