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International Journal of Bioprinting                                   3D-printed variable stiffness scaffolds











































































            Figure 9. Amount of sGAG between day 1 (D1) and day 21 (D21). (A) Amount of sGAG on D1 and D21. (B) Amount of sGAG retained in scaffolds
            or secreted into media at D21. (C) Amount of sGAG secreted into media between D1 and D21. (D) Amount of sGAG secreted into media for each
            scaffold between days 14 (D14) and D21, with increased secretion between D17 and D21. # denotes statistically significant differences between groups. *
            denotes statistically significant differences within groups. (E) Immunohistochemistry depicting collagen types I and II production in different hydrogel
            compositions. Scale bars: 100 µm. Abbreviations: CS: Chondroitin sulfate; GelMA: Gelatin methacryloyl; HA: Hyaluronic acid; HAMA: Methacrylated
            hyaluronic acid.


            Volume 10 Issue 4 (2024)                       509                                doi: 10.36922/ijb.3784
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