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Chen Y, et al.
the experiments and wrote the manuscript; and Ming-You
Shie contributed some detailed techniques.
Conflicts of Interest

The authors declare no competing financial interests.

References
1. Rengier F, Mehndiratta A, vonTengg-Kobligk H, et al., 2010,
3D Printing Based on Imaging Data: Review of Medical
Applications. Int J Comput Assist Radiol Surg, 5:335-41.
DOI 10.1007/s11548-010-0476-x.
2. Ventola CL, 2014, Medical Applications for 3D Printing:
Current and Projected Uses. P T, 39:704-11.
Figure 7. Alkaline phosphatase activity of human mesenchymal 3. Ciocca L, Mingucci R, Gassino G, et al., 2007, CAD/CAM
stem cells cultured on the MED610 substrate with various coatings
for 3 and 7 days. Ear Model and Virtual Construction of the Mold. J Prosthet
Dent, 98:339-43. DOI 10.1016/S0022-3913(07)60116-4.
its biocompatibility and be promoted the potential of 4. He J, Li D, Lu B, et al., 2006, Custom Fabrication of
cell bone differentiation. Previous studies reported that Composite Tibial Hemi-knee Joint Combining CAD/CAE/
PDA coatings on biological materials could facilitate CAM Techniques. Proc Inst Mech Eng H, 220:823-30. DOI
cell attachment and promote cell proliferation [22,23] . When 10.1243/09544119JEIM207.
HA was added in PDA coatings, the phenomenon of cell
calcium deposition was significantly promoted . This 5. Dai KR, Yan MN, Zhu ZA, et al., 2007, Computer-aided
[20]
means that the PDA/HA coatings can help to enhance Custom-made Hemipelvic Prosthesis Used in Extensive
osteogenic differentiation. In this study, the PDA/HA Pelvic Lesions. J Arthroplasty, 22:981-6. DOI 10.1016/j.
coatings were applied in MED610 objects. The results arth.2007.05.002.
show that the biocompatibility, cell morphology, and 6. Zopf DA, Hollister SJ, Nelson ME, et al., 2013, Bioresorbable
bone differentiation potential of the cells cultured on the Airway Splint Created with a Three-dimensional Printer.
MED610 objects with PDA/HA coating can be improved.
This study demonstrates that PDA/HA coating applications N Engl J Med, 368:2043-5. DOI 10.1056/NEJMc1206319.
also have similar functions on objects printed with 7. Cho HR, Roh TS, Shim KW, et al., 2015, Skull
[20]
MED610 materials to enhance their applicability. Reconstruction with Custom Made Three-dimensional
Titanium Implant. Arch Craniofac Surg, 16:11-16. DOI
5. Conclusion
10.7181/acfs.2015.16.1.11.
This study shows that using the PDA/HA coating 8. Jackson B, 2017, 3D Printed Hip Puts Teenager Back on her
added to the MED610 biomedical 3D printing material Feet. Available from: https://www.3dprintingindustry.com/
that has been used in the medical field can improve its news/materialise-3d-printed-hip-implant-gets-patient-back-
hardness, HA mineralization, cell proliferation, good
cell morphology, and the amount of ALP expression. feet-126139.
Therefore, the PDA/HA coating enhances the potential 9. Zhao X, Wang X, Sun Y, et al., 2018, Use of Three-dimensional
and development of MED610 materials for clinical Printing to Fabricate First a Pelvic Model and Then a Semi-
applications in orthopedics and dentistry. pelvic Prosthesis with Sacrum : A Case Report. Biomed J Sci
Tech Res, 4:1-4. DOI 10.26717/BJSTR.2018.04.001060.
Acknowledgments 10. Jacek B, Maciej P, Tomasz P, et al., 2018, 3D Printed Models
The authors acknowledge receipt of grants from the in Mandibular Reconstruction with Bony Free Flaps. J Mater
National Science Council grants MOST (107-2218-E- Sci Mater Med, 29:23. DOI 10.1007/s10856-018-6029-5.
468-005 – and 107-2622-E-468-002-CC2) of Taiwan and
China Medical University Hospital grants (CRS-106-021). 11. Gopinathan J, Noh I, 2018, Recent Trends in Bioinks for 3D
Printing. Biomater Res, 22:11. DOI 10.1186/s40824-018-
Authors’ Contributions 0122-1.

Yu-Fang Shen conceived the ideas and organized the main 12. Kaushik NK, Kaushik N, Pardeshi S, et al., 2015, Biomedical
content; Yi-Wen Chen and Hsin-Yuan Fang conducted and Clinical Importance of Mussel-inspired Polymers

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