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International Journal of Bioprinting Immunomodulatory bone repair by MBG/PCL

Figure 5. Differential immunomodulatory properties of MBG/PCL scaffolds. (A) Levels of RAW264.7 cell polarization gene expression promoted by the
scaffolds. (B1, B2) ALP staining of MBG/PCL scaffolds with BMSCs cultured in an MP-conditioned medium (B1) and the corresponding quantification
plot (B2). (C) Expression levels of osteogenesis-related genes (Alp, Opn, Runx2, Bmp2, Col1) in BMSCs cultured in MBG/PCL scaffolds with MP-
conditioned medium.

P500, and P800 groups were basically the same, and the P500, and P800: 70.01 ± 2.12%, 71.32 ± 4.83%, and 80.37 ±
pore diameters experienced gradual increase with the 1.91%, respectively). Meanwhile, the compressive strength
gradient of the initial design parameters, which were 241.33 of the scaffold decreases with increasing pore size. P200
± 29.79 μm, 535.17 ± 21.57 μm, and 794.80 ± 22.70 μm, has the best mechanical properties, while P500 decreases
respectively (Figure 7A). The fibers were crisscrossed with by about 4.90 MPa, and P800 shows the lowest compressive
each other, and the surface of the scaffold was brimming strength, which is 3.13 ± 1.09 MPa (Figure 7D).
with pores and MBG consistent with the previous scaffold.
According to Figure 7B and E, P500 has the largest contact 3.6 Effect of fiber thickness on the
angle, while P200 and P800 have almost similar contact immunomodulatory osteogenic properties of
angles of 86.53 ± 1.86° and 85.45 ± 0.47°, respectively. MBG/PCL scaffolds
Through Figure 7C, we can see that the larger the pore size As can be seen from the fluorescence staining results of
of the scaffolds, the higher the porosity (porosities of P200, Figure 8A, BMSCs were able to colonize scaffolds with

Volume 10 Issue 5 (2024) 329 doi: 10.36922/ijb.3551