Page 341 - IJB-10-5

International Journal of Bioprinting Immunomodulatory bone repair by MBG/PCL

Figure 10. Differential plots of proliferative activity and osteogenic properties of P200, P500, and P800 cells. (A, C) Live/dead staining and proliferative
activity of BMSCs of P200, P500, and P800. (B, D) Staining and quantification of ALP in P200, P500, and P800 co-cultured with BMSCs on day 7. (E)
Expression levels of osteogenic genes (Alp, Runx2, Opn, Col1, Bmp2) of P200, P500, and P800 co-cultured with BMSCs on days 7 and 14.

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topic. Particularly, bioceramics such as BGs are regarded proportion of glass to offset these shortcomings. 16,38 But,
as excellent bone-based restorative materials owing to their this is a detrimental move since the glass could degrade in
unique inorganic amorphous structure, bioactivity, and vivo and taken up by the body. 16,38 Therefore, in this study,
osseointegration properties. However, the dense pores and we synthesized SiO -CaO -P O ternary MBG by sol-gel
2
5
2
2
low specific surface area of common BGs and mesoporous method, and the dendritic nano-mesoporous structure
BGs limit the mechanical properties and bioactivity of the endowed the BGs with higher specific surface area (457.14
2
3
most scaffolds, and it is often necessary to increase the m /g) and pore volume (1.38 cm /g) (Figure 1). 3D printing

Figure 11. RAW264.7 cell polarization and immunomodulation of osteogenic properties promoted by P200, P500, and P800. (A) Levels of RAW264.7 cell
polarization gene expression induced by the scaffolds. (B1, B2) Staining and quantification plots of P200, P500, and P800 in MP-conditioned medium,
which mediated the expression of ALP in BMSCs. (C) Expression levels of osteogenic genes (Alp, Opn, Runx2, Bmp2, Col1) in P200, P500, and P800
scaffolds in MP-conditioned medium.

Volume 10 Issue 5 (2024) 333 doi: 10.36922/ijb.3551