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International Journal of Bioprinting 3D-printed plasma devices for decontamination

Figure 6. Differential effects of three different 3D-printed cold atmospheric plasma (CAP)-generating devices on the morphology of Pseudomonas aeruginosa
PAO1 cells visualized by transmission electron microscopy (TEM). As observed, increasing the duration of exposure to CAP damages the cell membrane,
leading to cell disruption and content leakage. TEM images of cells in (A) the unexposed control sample; (B) device 1, exposure for 1 min; (C) device 1,
exposure for 5 min; (D) device 1, exposure for 10 min; (E) device 2, exposure for 1 min; (F) device 2, exposure 5 min; (G) device 2, exposure for 10 min; (H)
device 3, exposure for 1 min; (I) device 3, exposure for 5 min; and (J) device 3, exposure for 10 min. Scale bars: 500 nm. Magnification: XXX.Will be done

min of exposure led to rupture of the cell membrane (Figure to CAP for 10, 30, 90, and 120 min. SARS-CoV-2
6G and J, respectively). Notably, complete leakage of the infectivity in permissive cells was completely eliminated
cell contents was visible after 10 min of CAP exposure via by optimized devices 2 and 3 after 90 min of exposure,
device 2 (Figure 6G). whereas original device 1 required 120 min (Figure 7A;
Table S1 in Supplementary File). Viral genome copy
3.4. CAP exposure reduces viral infectivity and numbers determined by qPCR demonstrated that the
compromises particle integrity optimized devices could effectively eliminate over 99.5%
We directly compared the virucidal effects of the three of SARS-CoV-2 RNA after 90 min, whereas the original
devices by exposing virus-contaminated parafilm squares device reduced the RNA level by 96% after only 120 min

Volume 10 Issue 5 (2024) 453 doi: 10.36922/ijb.3679