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International Journal of Bioprinting 3D model of neurogenesis in Alzheimer’s disease

Figure 5. Amyloid β (Aβ) oligomers modify the morphological aspects and induce oxidative stress in the 3D constructs. (A) Montage of stained
Z-projection, followed by 3D-plot reconstitution of one representative neurosphere stained with phalloidin/DAPI and LIVE/DEAD kit assay, respectively,
two days after bioprinting. (B) Oxidative stress assay in the neurospheres, using the CellROX reagent, on days 2 and 3 after bioprinting. (C) Quantification
of reactive oxygen species (ROS) in the neurospheres of the constructs, on days 2 and 3 after bioprinting ( p < 0.5; p < 0.001). (D) Scanning electron
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microscopy (SEM) micrographs of the surface morphology of constructs without and with Aβ oligomers three days after printing. Black arrows indicate
neurospheres immersed in a smooth construct surface. Red arrows indicate neurospheres within a rough construct under Aβ oligomers exposure. Scale
bars: 20 µm.
Volume 10 Issue 5 (2024) 517 doi: 10.36922/ijb.3751