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International Journal of Bioprinting 3D model of neurogenesis in Alzheimer’s disease

Figure 6. Amyloid β (Aβ) oligomers in 3D constructs induce neural stem cell (NSC) differentiation three days after bioprinting. (A) Construct with
neurospheres. (B) Construct with neurospheres + Aβ oligomers. The micrographs indicate a sequence of neurosphere staining, where blue: DAPI (nuclei),
green: MAP-2 (mature neurons), and red: Nestin (NSCs), followed by a Z-projection of MAP-2 and Nestin cells. Scale bars: 20 μm. The 3D plots
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reconstitute the surface of the constructs, where stained cell distribution can be observed from a 3D perspective. (C) Quantification of Nestin and MAP-2 +
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cells in constructs with and without Aβ oligomers (**** p < 0.0001; × indicates maximum or minimum values; and ם indicates the mean).
Volume 10 Issue 5 (2024) 518 doi: 10.36922/ijb.3751