Page 84 - IJB-10-5

International Journal of Bioprinting dECM bioink for 3D musculoskeletal tissue reg.

Table 2. Decellularization and digestion strategies used to produce decellularized extracellular matrix (dECM)
Tissue Subject Decellularization method Digestion method Ref.
Heart Porcine SDS; Triton-X 100; deoxycholate Acetic acid; pepsin 119
Porcine 1% SDS; 1% Triton-X 100 Acetic acid; pepsin 120, 38, 121

Porcine 1% ABAM; 0.01 mM PMSF DNase; 0.25% trypsin-EDTA 68
Human HSS; hypotonic solution; 1% SDS Acetic acid; pepsin 122
Porcine 1% SDS; 1% Triton-X100 5 M Urea; 2 M thiourea; 50 mM DTT; 0.1% 41
SDS in PBS
Porcine 1% SDS; 1% DNase; 1 U/mL RNase; Tris-HCl Pepsin; acetic acid 123
Porcine 1% SDS; 50 U/mL DNase; 1 U/mL RNase A; Tris– 10% Pepsin; 0.5 M acetic acid 124
HCl; 0.5% Triton X-100; 1% peracetic acid; 4%
ethanol
Porcine 1% SDS; 1% Triton X-100; DPBS containing 1% 0.1% Pepsin; 0.5 M acetic acid 125
antibiotic/antimycotic; DNase
Skeletal Porcine 0.25% Trypsin-EDTA; 2% SDS 3 mg/mL Pepsin; 0.1 M HCl 126
muscle
Porcine 0.25% Trypsin-EDTA; 0.5% SDS; 0.5% TritonX-100; 3 mg/mL Pepsin; 0.5 M AA; 0.1 or 0.02 M 127
0.1% peracetic acid; 4% ethanol HA
Porcine 0.25% Trypsin; PBS; 50 U/mL type I DNase; 1 U/mL 0.25% Trypsin; PBS; vigorous agitation 128
RNase; 10 mM Tris–HCL
Tendon Porcine 0.05% Trypsin; 0.02% EDTA 0.01 M HCl; pepsin; 15% (w/v) NaCl 129
Skeleton Human 0.05% trypsin; 0.02% EDTA; 5% CO ; 100 IU/ 1 mg/mL Pepsin; 0.01N HCl 130
2
mL DPBS-containing penicillin; 100 µg/mL
streptomycin
Goat 3.5% PMSF; 1% Triton X-100; protease inhibitor 50 U/mL DNase; 1 U/mL RNase; 0.1 mol 131
cocktail (0.1% EDTA; 3.5% PMSF; Tris–HCl) HCl; 1 mg pepsin; 1 mol NaOH
Porcine 0.05% Trypsin; 0.02% EDTA 0.01 M HCl; 1 mg/mL pepsin; 15% (w/v) 132
NaCl
Rabbit Freeze-thaw cycles; 0.1% SDS; 0.1% EDTA; DNase I; - 64
Tris–HCl; 50 mg/mL BSA buffer
Porcine Freeze-thaw cycles; 1% Triton X-100; 1% SDS 200 U/mL DNase I; 0.5 M acetic acid; 30 133
mg pepsin
Cartilage Porcine Freeze-thaw cycles; 0.25% trypsin-PBS; 3% sodium 0.5 M Acetic acid; pepsin; 10 M NaOH 134
deoxycholate; 50 U/mL DNase
Calf Freeze-thaw cycles; HSS; 0.01% (v/v) Triton X-100; 0.1 M HCl; 1 mg/mL pepsin; 1 M NaOH 135
1% (v/v) SLS; 40% (v/v) ethanol
Porcine Freezing-thaw cycles; 1% Triton X-100; protease 10 mM Tris–HCL; 1 mM EDTA 136
inhibitor; HBSS; 200 U/mL DNase
Goat 0.1% (w/v) EDTA; 3.5% (w/v) PMSF; 1% Triton 50 U/mL DNase; 1 U/mL RNase for 12 h; 25
X-100; Tris–HCl; protease inhibitor cocktail 0.1 mol HCl; 1 mg pepsin; 1 M NaOH
Rat 1:1 of 0.25% trypsin to 0.2% type I - 65
collagenase; 0.05% EDTA; 3% SDS; 3% Triton X-100
Rabbit 1% (w/v) SDS/PBS; 0.1% (w/v) EDTA/PBS Pepsin; 0.01 M HCl; 0.1 M NaOH 137
Abbreviations: AA, acetic acid; ABAM, antibiotic-antimycotic; BSA, bovine serum albumin; DPBS, Dulbecco’s phosphate-buffered saline; DTT,
Dithiothreitol; EDTA, ethylenediamine tetra acetic; HA, hydrochloric acid; HBSS, Hank’s balanced salt solution; HSS, hypertonic salt solution; PBS,
phosphate-buffered saline; PMSF, phenylmethylsulphonyl fluoride; SDS, sodium dodecyl sulfate; SLS, sodium lauroyl sarcosine.

Volume 10 Issue 5 (2024) 76 doi: 10.36922/ijb.3418