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HRP plus glucose-mediated bioprinting
material due to its ability to promote cell adhesion 18H01797 and Grand-In-Aid for JSPS Fellows
and proliferation. The lattice-shaped construct 18J11601.
obtained using the selected ink was soaked in a
solution containing rhodamine-labeled Gel-Ph Conflicts of interest
(1.0 w/v%, Rho-Gel-Ph) right after printing There are no conflicts of interest to declare.
(Figure 6A). Due to the remaining HRP and
glucose, Gel-Ph can be cross-linked with non- References
cross-linked Ph moieties in the hydrogel. After
1 day of soaking, the entire surface of the 1. An J, Teoh JEM, Suntornnond R, et al., 2015, Design and 3D
lattice-shaped construct had a strong signal of Printing of Scaffolds and Tissues. Engineering, 1(2):261–8.
red fluorescence derived from Rho-Gel-Ph, DOI 10.15302/J-ENG-2015061.
which indicates the successful coating process 2. Liu F, Mishbak H, Bartolo P, et al., 2019, Hybrid
(Figure 6B). Subsequently, 10T1/2 cells were Polycaprolactone/Hydrogel Scaffold Fabrication and In-
seeded on the construct to confirm the switched process Plasma Treatment Using PABS. Int J Bioprint,
culture surface. As shown in Figure 6C, the cells 5(1):174. DOI: 10.18063/ijb.v5i1.174.
adhered to and elongated on the entire surface of 3. Yang Y, Wang G, Liang H, et al., 2019, Additive Manufacturing
the construct. These results demonstrate that it is of Bone Scaffolds. Int J Bioprint, 5(1):148. DOI: 10.18063/
possible to switch non-cell-adhesive surface of the ijb.v5i1.148.
printed construct to cell-adhesive surface with a 4. Zhuang P, Sun AX, An J, et al., 2018, 3D Neural Tissue
simple procedure. Moreover, the ability to modify Models: From Spheroids to Bioprinting. Biomaterials,
the surface with desired materials through the 154:113–33. DOI: 10.1016/j.biomaterials.2017.10.002.
proposed method enables to design functionalized 5. Mironov V, Trusk T, Kasyanov V, et al., 2009, Biofabrication:
3D construct for individual applications. A 21 Century Manufacturing Paradigm. Biofabrication,
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4 Conclusions 6. Mir TA, Nakamura M, Iwanaga S, et al., 2019, Biofabrication
Offers Future Hope for Tackling Various Obstacles and
We demonstrated the feasibility of utilizing Challenges in Tissue Engineering and Regenerative Medicine:
glucose-mediated enzymatic hydrogelation for A Perspective, Int J Bioprint, 5(1):153. DOI: 10.18063/ijb.
extrusion-based bioprinting. The cross-linking v5i1.153.
of Alg-Ph and CNF-based bioink through HRP- 7. Ng WL, Chua CK, Shen YF, et al., 2019, Print Me an Organ!
catalyzed reaction that consumes H O generated Why We are not There Yet. Prog Polym Sci, 97:101145. DOI:
2
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by HRP and glucose enabled to print 3D cell- 10.1016/j.prog-polymsci.2019.101145.
laden construct with good shape fidelity. The 8. Lee JM, Sing SL, Zhou M, et al., 2018, 3D Bioprinting
cell-laden construct was successfully cultured Processes: A Perspective on Classification and Terminology.
for 7 days without collapsing. In addition to the Int J Bioprint, 4(2):151. DOI: 10.18063/ijb.v4i2.151.
potency of printing with living cells, it was also
demonstrated that the printed construct can be 9. Murphy SV, Atala A, 2014, 3D Bioprinting of Tissues and
used as a scaffold for cell culture after coated with Organs. Nat Biotechnol, 32(8):773–85. DOI: 10.1038/
nbt.2958.
Gel-Ph through the same cross-linking method. 10. Du X, 2018, 3D Bio-Printing Review. Mater Sci Eng,
Overall, the proposed method advances the ability 301(1):012023. DOI: 10.1088/1757-899X/301/1/012023.
of bioprinting with living cells with a mild and cell 11. Heinrich MA, Liu W, Jimenez A, et al., 2019, 3D Bioprinting:
compatible cross-linking.
From Benches to Translational Applications. Small,
Acknowledgments 15(23):1805510. DOI: 10.1002/smll.201805510.
12. Lee JY, An J, Chua CK, et al., 2017, Fundamentals and
This work was supported by the Japan Society Applications of 3D Printing for Novel Materials. Appl Mater
for the Promotion of Science (JSPS) KAKENHI Today, 7:120–33. DOI: 10.1016/j.apmt.2017.02.004.
Grant Numbers 15H04194, 16H02423, 17H03472, 13. Nakamura K, Nishiyama Y, Henmi C, et al., 2008, Ink
50 International Journal of Bioprinting (2020)–Volume 6, Issue 1
