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Fibrin-based Bioinks

Table 3. Bioprinting with fibrin-based bioinks.
Components Bioprinting Origin Cells Outcomes Ref
Fibrinogen Cross-linking Additives
agents
60 mg/ml Thrombin 50 Furfuril-gelatin Extrusion- n/a iPSC-derived Printed structures [89]
U/ml 155 mg/ml based cardiomyocytes were porous and
CaCl2 80 RB showed good
mM stability
Cells remained
viable, proliferated,
and expressed
cardiac marker
(troponin I)
30 mg/ml Thrombin n/a Gelatin 35 mg/ml Extrusion- n/a bladder Cells maintained [94]
Hyaluronic acid based urothelial cells, high viability
3 mg/ml bladder smooth for a week after
muscle cells printing, actively
proliferated and
expressed specific
biomarkers
50 mg/ml NaCl 150 Hyaluronic acid Extrusion- Bovine primary Cells were viable [36]
mM 4 mg/ml based Schwann cells and proliferated
CaCl2 5 mM Factor XIII 1 U/ml Directed cell
PVA Aprotinin 0.5 alignment was
1.4%w/v mg/ml observed
Thrombin 50
U/ml
10 % w/v Thrombin Gelatin 5 % w/v Extrusion- Bovine HUVECs, EC-MSC distance [93]
100 U based MSCs defining cell-cell
communication
regulates
angiogenesis
20 mg/ml Thrombin 50 Gelatin 30 mg/ml Extrusion- n/a neonatal rat Bioprinted [90]
U/ml based ventricular constructs
Aprotinin cardiomyocytes contracted,
20 µg/ml were formed
Hyaluronic acid by aligned and
3 mg/ml Glycerol electromechanically
10% coupled cells and
responsive to drugs
20 mg/ml Thrombin Gelatin Extrusion- Bovine primary Heat-treated [95]
2000 U/ml 7.5 % w/v based, rotary neonatal gelatin increased
human dermal fibrinogen-based
fibroblast bioink printability
Tensile mechanical
properties of
printed constructs
induced the rise in
circumferential and
axial elastic moduli

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34 International Journal of Bioprinting (2020)–Volume 6, Issue 3