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Soman and Vijayavenkataraman
Figure 1. Bioprinting of induced pluripotent stem cell (Ipsc)-derived tissues for regenerative medicine,
disease modeling, and drug testing. Adult somatic cells are collected from a donor or a patient; the cells
are reprogrammed to iPSCs and differentiated to specialized cells.
Figure 2. Reprogramming methods used to generate induced pluripotent stem cell.
viral vectors [1,2,31-34] . It is essential to do the quality for transcription factor delivery. Recent methods of
control of the iPSC lines created using viral reprogramming use Sendai Virus (SeV) particles
vectors using whole-genome single nucleotide and episomes. SeV particles are used to transduce
polymorphism array and karyotyping to verify the the reprogramming genes: Oct4, Sox2, Klf4, and
genomic integrity. c-Myc . SeV reprogramming offers the absence
[8]
2.2 Non-integrating vectors of viral sequences in the host cell lines and is an
efficient method to induce pluripotency . In 2009,
[35]
To reduce the risk of unwanted genetic perturbations, Yu et al. reported the episomal reprogramming in
there was introduction of non-integrating methods human cells where Epstein-Barr virus–derived
International Journal of Bioprinting (2020)–Volume 6, Issue 4 61
