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International Journal of Bioprinting                         Innovative manufacturing of ω-3-enriched chocolate




            differences in the b* value.  According to these findings,   suggest that different types of loading, such as ω-3-mixed
            the L* value was associated as a color indicator since the   chocolate and ω-3-SA  MP, could decrease strain values
            a* and b* values did not give any distinction among the   compared to pure chocolate scaffolds.
            chocolate samples (p > 0.05). Similar results were obtained
            by Erunsal et al. 47                               3.7. Analysis of thermal properties
                                                               Differential scanning calorimetry (DSC) was used to
            3.6. Mechanical analysis                           examine the melting temperatures (T ) of three different
                                                                                             m
            Products, such as biscuits, cakes, and cookies are   chocolate groups that were evaluated in this study.
            characterized by flavor and texture when consumed; fat is
            one of the factors that affects texture.  The texture of the   Figure  7  displays the DSC thermogram values of pure
                                          54
            samples was evaluated by conducting a compression test.  chocolate, ω-3-mixed chocolate, and ω-3-SA MP-coated
                                                               chocolate. The  T   and  T   values of the chocolate
                                                                                     endset
                                                                             onset
               The influence of different loading types (mixing and   samples were found to be between 25.26–28.88°C  and
            coating processes) of ω-3 on chocolates in relation to   36.07–42.19°C, respectively. The ΔH values varied between
            their  mechanical  properties  is  described  in  Table  2.  A
            compressive strength value of 0.65 MPa was obtained for   Table 2. Compressive testing results for pure, ω-3-mixed,
            the pure chocolate, along with a strain value of 81.73%.   and ω-3-sodium alginate (SA) microparticle (MP)-coated
            In contrast, the incorporation of SA/ω-3 solution into the   chocolate scaffolds.
            3D-printed pure chocolate scaffolds led to an increase in
            the compressive strength to 0.82 MPa, with a decrease in   Scaffold  Compressive strength   Strain (%)
            the strain value to 61.08%. Keshavarz et al. indicated that              (MPa)
            the mechanical properties of 3D structures improved after   Pure chocolate  0.65  ± 0.025  81.73  ± 3.377
                                                                                                     c
                                                                                     a
            coating with alginate.  As noted in the compression test,   ω-3-mixed chocolate  0.60  ± 0.003  75.98  ± 7.445
                             55
                                                                                     a
                                                                                                     c
            the incorporation of alginate into pure chocolate scaffolds   ω-3-SA MP coated   0.82  ± 0.064  61.08  ± 8.496
                                                                                     b
                                                                                                     b
            via coating was associated with an increase in compressive   chocolate
            strength. The addition of ω-3 to the melted chocolate   Note: Data are expressed as the mean ± standard deviation of triplicate
            solution  resulted  in  a  reduction  in  both  compressive   measurements. Means with different letters in the same column are
            strength (0.60 MPa) and strain value (75.98%). The results   significantly different (p < 0.05).































            Figure  7. Differential scanning calorimetric (DSC) graph of pure chocolate, ω-3-mixed chocolate, and ω-3-sodium alginate (SA) microparticle
            (MP)-coated chocolate.


            Volume 10 Issue 6 (2024)                       382                                doi: 10.36922/ijb.3969
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