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International Journal of Bioprinting Nanomaterial-bioinks for DLP bioprinting

Figure 5. (A) DNA quantification of human mesenchymal stem cells (hMSCs) in printed gelatin methacrylamide (GelMa)-based bioinks after 7 and
14 days (n = 4 donors). (B–E) Results of DNA quantification of hMSCs in printed GelMa-based bioinks for four different donors after 7 and 14 days
(n = 3 technical replicates). Two-way ANOVA was used for statistical evaluation; *p < 0.05, **p < 0.01; ***p < 0.001; ****p < 0.0001. Abbreviations: ANOVA:
Analysis of variance; BB: Brilliant Black; CaP: Calcium phosphate; GO: Graphene oxide.

The amount of DNA in GelMa-based constructs was on average, the highest DNA content at day 14 compared
assessed on days 7 and 14 to analyze the impact of the to the nanomaterial-modified variants, as well as a
nanomaterial and the temporal development of cell growth significant increase in the DNA amount over time, thus
over the tested time frame. GelMaBB samples displayed, indicating cellular proliferation in GelMaBB (Figure 5A).

Volume 10 Issue 6 (2024) 481 doi: 10.36922/ijb.4015