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International Journal of Bioprinting Nanomaterial-bioinks for DLP bioprinting

Figure 4. Confocal microscopy of human mesenchymal stem cells (hMSCs) encapsulated in the printed constructs: commercial ink (A, E, I, and M),
GelMaBB (B, F, J, and N), GelMaBB-CaP (C, G, K, and O), and GelMaGO (D, H, L, and P). (A–H) Live/dead staining with calcein AM (green) and
propidium-iodide (red) after 7 (A–D) and 14 (E–H) days. (I–P) Staining with Phalloidin TRITC (red) and Hoechst 33258 (blue) after 7 (I–L) and 14 (M–P)
days. Scale bars: 250 µm. Abbreviations: GelMa: Gelatin methacrylamide; BB: Brilliant Black; CaP: Calcium phosphate; GO: Graphene oxide.

3.5. Influence of nanomaterial-modified bioinks on donors of hMSC (Figure 5). The reference GelMa bioink
cell number and proliferation (commercial), however, had the lowest DNA amount in
To assess the cellular performance in the different pilot studies in accordance with the morphological data as
bioinks quantitatively, we determined the DNA amount described above. Thus, we focused on the GelMaBB bioink
in the DLP-printed constructs over time and for several and its modifications in the upcoming experiments.

Volume 10 Issue 6 (2024) 480 doi: 10.36922/ijb.4015