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Al-Tamimi
           linear  hexahedral  elements  and  meshed  with  different   by the mesh density [16,18] .  The  effect  of  mesh  size  on
           number  of  elements  (5000  elements,  10,000  elements,   the  optimization  process  is  shown  in  Figure  3 and
           25,000  elements,  50,000  elements,  75,000  elements,   the  corresponding  equivalent  stiffness  is  presented  in
           100,000 elements, 250,000 elements, 350,000 elements,   Figure 4. Coarse mesh results in less material removal
           and 400,000 elements).

           3.4. Stress shielding analysis
           The  stiffness  of  each  plate,  for  different  loading
           conditions, is the main criteria to determine possible stress
           shielding  problems. In all  cases, the optimized  plates
           were considered homogeneous and isotropic. Based on
           the numerical finite element analyses and their loading
           and boundary conditions in Table 2, the corresponding
           equivalent stiffness of each plate was determined . For
                                                    [12]
           the  compression case  (similar  to  combined  load  case),
           the  equivalent  stiffness  was  calculated  considering  the
           longitudinal reaction uniaxial force (RF ) in kilonewton
                                            xx
           (kN) along the X-axis applied on one end of the bone
           plate and constraining the opposite side, as follows:
                                      RF
                                K Te  =  D xx xx       (3.1)

               where KTe is the equivalent stiffness in kN/mm and
           Dxx is the resulted displacement in mm. In the bending case,
           the equivalent bending stiffness was calculated according to
           the following equation (British Standard 1991) :
                                                [15]
                              ( 4h +  2  12hς  ς +  2 ) Sh
                         K =          24               (3.2)
                           B
               where K  is the equivalent bending stiffness in N.m ,
                                                         2
                      B
           h is the distance between the load and support points, is
           the distance between the load points, and S is the slope of
           the load-deflection curve.
               The  equivalent  elastic  bending  modulus  was
           measured as follows (ASTM Standard 2017) :
                                                [17]
                                   3
                                     /
                         E =  0.17L S bd 3             (3.3)   Figure 3. Mesh dependency - plate redesigns for different number
                           B
                                                               of elements.
               where E  is the equivalent bending elastic modulus
                      B
           in GPa, L is the support span, b is the plate’s width, and d
           is the plate’s thickness.
               For  the  torsion  case,  the  equivalent  stiffness  was
           determined considering the reaction moment across the
           X-axis (T ) applied on one end of the plate, constraining
                   xx
           the other end. The resulted angle of twist along the X-axis
           direction  (φ )  was  recorded  and  the  torsional  stiffness
                     xx
           was determined as follows:
                                      T
                                K   =  xx              (3.4)
                                  Tr
                                      ϕ xx
               where K  is the torsional stiffness in Nmm/Rad.
                      Tr
           4. Results and discussion
           Despite utilizing mesh-dependency filtering techniques,
           TO  in  commercial  software  appears  to  be  affected   Figure 4. Equivalent stiffness values as a function of mesh size.
                                       International Journal of Bioprinting (2021)–Volume 7, Issue 3       157
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