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REVIEW ARTICLE

Developments and Opportunities for 3D Bioprinted

Organoids

Ya Ren 1,2† , Xue Yang 1,2† , Zhengjiang Ma , Xin Sun , Yuxin Zhang , Wentao Li , Han Yang ,
1†
3
1
1
4
Lei Qiang , Zezheng Yang , Yihao Liu , Changxu Deng , Liang Zhou , Tianchang Wang ,
1
5
1
1
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6
Jingsheng Lin , Tao Li *, Tao Wu *, Jinwu Wang *
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9
1,4
1 Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital
Affiliated Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Rd, Shanghai 200011, China
2 Southwest JiaoTong University College of Medicine, No. 111 North 1 Section of Second Ring Road, Chengdu 610036, China
st
3 Department of Rehabilitation Medicine, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University
School of Medicine, Huangpu District, Shanghai 200011, China
4 Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Rd, Shanghai
200030, China
5 Key Laboratory of Advanced Technology of Materials, Ministry of Education, School of Materials Science and
Engineering, Southwest Jiaotong University, Chengdu 610036, China
6 Department of Orthopedics, The Fifth People’s Hospital of Shanghai, Fudan University, Minhang District, Shanghai
200240, P. R. China
7 Center for Medicine Intelligent and Development, China Hospital Development Institute, Shanghai Jiao Tong University,
Shanghai 200020, China
8 Department of Information Technology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
9 Department of Orthopaedics, Xinhua Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai
200092, P. R. China
10 Shanghai University of Medicine and Health Sciences, Shanghai 200120, China
† These authors contributed equally to this work.
Abstract: Organoids developed from pluripotent stem cells or adult stem cells are three-dimensional cell cultures possessing certain
key characteristics of their organ counterparts, and they can mimic certain biological developmental processes of organs in vitro.
Therefore, they have promising applications in drug screening, disease modeling, and regenerative repair of tissues and organs.
However, the construction of organoids currently faces numerous challenges, such as breakthroughs in scale size, vascularization,
better reproducibility, and precise architecture in time and space. Recently, the application of bioprinting has accelerated the process
of organoid construction. In this review, we present current bioprinting techniques and the application of bioinks and summarize
examples of successful organoid bioprinting. In the future, a multidisciplinary combination of developmental biology, disease
pathology, cell biology, and materials science will aid in overcoming the obstacles pertaining to the bioprinting of organoids. The
combination of bioprinting and organoids with a focus on structure and function can facilitate further development of real organs.
Keywords: Bioprinting; Organoid; Tissue engineering; Organ development

*Correspondence to: Tao Li, Department of Orthopaedics, Xinhua Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai
200092, P. R. China; litaoxyeyy@sina.com; Tao Wu, Shanghai University of Medicine and Health Sciences, Shanghai 200120, China; wutao0324@
shsmu.edu.cn; Jinwu Wang, Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People’s
Hospital Affiliated Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Rd, Shanghai 200011, China; wangjw-team@shsmu.edu.cn

Received: April 3, 2021; Accepted: May 8, 2021; Published online: June 28, 2021
(This article belongs to the Special Section: Bioprinting of 3D Functional Tissue Constructs)
Citation: Ren Y, Yang X, Ma Z, et al., 2021, Developments and Opportunities for 3D Bioprinted Organoids. Int J Bioprint,
7(3):364. http://doi.org/10.18063/ijb.v7i3.364

© 2021 Ren, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/
licenses/by/4.0/), permitting distribution and reproduction in any medium, provided the original work is cited.
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