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Wang, et al.
           2.4. 3D printing for solid modules                  2.6. Evaluation of osteotomy

           The  intertrochanteric  region,  distal  femur,  and  rotation   The  intermediate  fragments  after  osteotomy  were
           control lever modules were imported into 3D printer to   collected and the mass was measured using an electronic
           manufacture  anatomical  osteotomy  modules  for each   balance  (accuracy:  0.001  g). The  volume  of fragments
           component (Figure 5).                               was measured  according  to  drainage  method  with  a
                                                               50-ml graduated measuring cylinder (accuracy: 0.1 ml)
           2.5. Assembly and osteotomy                         and 500-ml measuring cup.
           Fourteen composite Sawbones were used for osteotomy.   2.7. Statistical analysis
           A  senior biomechanical specialist performed osteotomy
           according to a standard method: Placing guide needle in   Shapiro–Wilk test was used to determine the normality
           Sawbones in the intramedullary main nail guides-guided   of continuous data. The mass and volume of intermediate
           procedure, expanding medullary cavity to appropriate   fragments  were  presented  as the  mean  ±  standard
           depth, and inserting and subsequently removing      deviation  or median (interquartile  range). Deviations
           intramedullary nail. Then, distal femur osteotomy modules   from the average of volume and mass that  measure
           were assembled at the distal femur after removal of distal   individual differences between individual and the mean
           femoral condyle. The intertrochanteric region osteotomy   of population  of every fragment were calculated  and
           modules were assembled at the proximal femur and a   visualized  using one-dimensional histogram.  GraphPad
           horizontal band-saw was used to create AO/OTA 31 A2.3   Prism  (8.3.0  version)  and  “ggplot2”  package  in  R
           unstable intertrochanteric fracture models (Figure 6).  software (3.6.3 version) were used for data analysis and
                                                               visualization, respectively.
           A                      B
                                                               3. Results
                                                               Satisfactory  osteotomies  of all  composite  Sawbones
                                                               were achieved (Figure 7). The mean volume and mass
                                                               of intermediate  fragments were 21.0 ± 1.5 mm  and
                                                                                                          3
                                                               19.0  ±  1.2  g,  respectively. Range  of  deviation  from
                                                               average of volumes was −1.9 – 2.8 mm and most of these
                                                                                               3
           C                      D
                                                               deviations fall within the range of −1.4 – 2.1 mm . Range of
                                                                                                      3
                                                               deviation from average of mass was −2.0 – 1.6 g and most
                                                               of these deviations fall within the range of −1.4 – 1.6 g.
                                                               One-dimensional histogram of deviation from average
                                                               shows the precise and stable osteotomy performed based
                                                               on modules accordingly (Figure 8).

                                                               4. Discussion
           Figure 4. Osteotomy models were designed in 3-Matic. (A and B)
           Intertrochanteric region models. (C and D) Distal femur models.  Precise and shape-matching  osteotomy models are
                                                               determinants  of the  experimental  homogeneity  in  the
                                                               assessment of orthopedic implants mechanical properties.




















           Figure  5.  3D printer was  used to print intertrochanteric  region
           osteotomy modules.                                  Figure 6. Horizontal band-saw was used to create fracture models.

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